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Why is isolated DNA suspended in TE buffer only instead of any buffer like phosphate buffer or so?
Wiki User
∙ 14y ago
Tris EDTA buffer inhibits nucleases that will degrade the DNA, by chelating cations required by the enzymes.
Phosphate buffer offers no such protection against degradation.
Wiki User
∙ 14y ago
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Which buffer system acts the fastest?
bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.
What is the function of PBS buffer?
The main function of PBS (phosphate buffer saline) is to maintain the pH of the solution. by Himanshu Goel Jaypee University of Information Technology
How does the phosphate buffer system help in maintaining the pH of our body?
The phosphate buffer system consists of two ions: dihydrogen phosphate ions and hydrogen phosphate ions. When the number of hydrogen ions in a body's bloodstream increases (pH drops), hydrogen phosphate ions accept hydrogen ions in order to maintain the equilibrium between the concentration of hydrogen and hydroxide ions within the bloodstream. When the number of hydrogen ions in the bloodstream decreases (pH increases), the resulting dihydrogen phosphate ions release hydrogen ions in order to increase the number of hydrogen ions in the blood.
What is the most important buffer in extracellular fluids?
Bicarbonate Buffer System (only important ECF buffer)
Water used to prepare gel instead of tbe buffer?
Dna is soluble in water
If use sodium phosphate buffer instead of potassium phosphate buffer?
its ok to use
How to prepare phosphate buffer pH 5.8?
preparation of 5.8 ph phosphate buffer
How you prepare 10 mM phosphate buffer from 0.5 M phosphate buffer?
By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer
How I can prepare 5 mM phosphate buffer?
5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).
Which buffer system acts the fastest?
bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.
What is the difference between phosphate buffer and phosphate buffered saline?
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
What are different types of buffering system?
1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system
How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?
Without phosphate you can't.
What happens when you add NaCl salt in it's solid state to a phosphate buffer system?
Bonds to the phosphate
Why DNA isolated stored in buffer not water?
So as to maintain a favourable pH for the isolated DNA
Why is the phosphate buffer made up by using the Henderson hasselbalch equation not the expected PH?
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?
What is the principle buffer in the body?
A buffer is something that regulates or maintains the pH in the body. In the human body, carbonate is the main buffer in the blood and phosphate is the main buffer within cells.
