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Q: Why is it Important to leave stain on a smear for a period of time?
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Can you leave stain on your wood panels without wiping it off?

Yes, if it's a gel stain. However, if it's a penetrating stain it will not dry correctly. If you try to clean it, it will smear. If you put a clear topcoat on it, the topcoat will smear the excess stain.


What is the purpose of covering the smear with bibulous paper when performing the endospore stain?

This paper will aid in keeping the stain over the bacterial smear.


What is another word for smudge?

Smear


What is the purpose of staining the smear in malachite green during spore staining?

the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.


What is the function of methanol in the wright's stain solution?

to fixed the blood smear


Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?

smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.


What is used to make the cells in the blood smear more easily observable?

Wright's stain


Why use methanol to fix smear during giemsa staining?

because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.


Give the reason for passing bacterial smear through the flame before staining?

It dries the smear and fixes the cells to the slide


What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?

A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.


What is another name for a tzanck preparation?

Tzanck preparation is also called a Tzanck smear, herpes stain for inclusion bodies, or inclusion bodies stain.


What happens when you leave a stain on the bacterial smear for too long?

If the bacterial cell are under stained then they will loose the stain of dye when wash with alocohol or may be simply by water which then cause a problem in identifying the cell type as e.g. in case of gram staining if the cell do not stain properly with methylene blue then they will loose the stain when washed and will counter stain with saffranin so the gram +ve will show the gram -ve colour.