When the amount of DNA iolated from cells is of a very small quantity and we are interested in studying only a specific stretch of DNA, it needs to be amplified
DNA amplification means making millions of copies of a very specific stretch of the DNA
After amplification, it becomes easier to analyze DNA using a variety of analytical techniques like gel electrophoresis
why is it important to dna extracted from the tissues
Because the amplification process has the ability to increase the amount of DNA required for a particular experiment
A gene may represent only a millionth of the cell's DNA
Yes you do. Chicken meat are made of tissues. Tissues are made from cells and the cells contain DNA.
Template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
What do you really want to ask? template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
Since the point of PCR is to amplify a copy of DNA, it would result in many copies of DNA that you want to study.
The PCR reaction can be used to amplify DNA from all three sources mentioned. PCR relies on the use of short stretches of DNA that are 6 - 12 bases long to attach to the target DNA (the source where the DNA is coming from) so that the polymerase enzyme can make copies of the target DNA. As long as these primers are available (they can be commercially purchased in many cases), PCR can be carries out on fetal cell DNA and viral DNA. Fossil DNA however, may have undergone degradation. DNA has to be of a certain purity for PCR to work. If the fossil DNA had degraded or broken down, PCR cannot be carried out.
Please read about PCR, it is a tool to amplify any kind of DNA/gene
polymerase chain reaction (PCR)
PCR
Poly merase chain reaction
DNA
Using the Polymerase Chain Reaction, scientists can amplify even the smallest amount of DNA, by constant cycles of separation and replication.
Yes you do. Chicken meat are made of tissues. Tissues are made from cells and the cells contain DNA.
Template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
The two main purposes for analyzing VNTR from DNA fingerprints are matching tissues and inheritance - This is True
What do you really want to ask? template DNA is a DNA you want to amplify. So you should know what you are amplifying before a PCR or you can make it by sequencing your PCR product.
PCR or polymerase chain reaction is a method to amplify a fragment of DNA. PCR reaction contains template DNA, primers, dNTPs, polymerase enzyme, buffer and water. The thermocycler manage the heat and time to synthesize DNA (denaturation, annealing and extension). The main application is one can amplify the gene or DNA of interest to millions of copies by using this prior to cloning.
Real time PCR is uded to amplify and simultaneously quantify a targeted DNA molecule.