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Why is it important to inactivate the shrimp alkaline phosphatase before ligating your EcoRI cut pBluescript plasmid to a foreign DNA fragment?
Wiki User
∙ 12y ago
because you need the phosphate of the foreign DNA fragment for ligation. If not inactivated before you add the fragment, SAP will imediately remove the phosphate form your fregment too leaving you with two dephosphorylated fragments that are not easy to ligate..
Wiki User
∙ 12y ago
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What is a water break free surface?
has to do with alkaline dip tanks.. Water break free surface is a 10 second retention of water on a surface that has been cleaned and rinsed of its alkaline.
What is the difference between alkaline and copper batteries?
THere is no difference i have answered this question i am the best
What is inside of expensive batteries last longer than cheaper batteries?
the most common non rechargeable battery is alkaline batteries. the more expensive batteries contain more lithium. the lithium batteries last a lot longer. you can also buy heavy duty batteries. although it sounds like they should last longer..they don't. heavy duty batteries are actually a step down from alkaline.
Why is a section of the battery referred to as a lead-acid cell?
Alkaline batteries are named for their highly alkaline electrolyte solution which is potassium hydroxide (aka caustic potash, aka "traditional" lye) mixed with water. Lead-Acid batteries are similarly named in-part after their highly acidic electrolyte solution consisting of 35% sulfuric acid and 65% water.
Did Lewis urry make a profit on the alkaline battery?
No. He worked for Ever-Ready; his patent was filed through the company. Therefore, he made no direct profit. He may have gotten a raise, call it what you want.
How is alkaline phosphatase testing performed?
Alkaline phosphatase is measured by combining the person's serum with specific substances with which alkaline phosphatase is known to react.this reaction is measured.the amount of alkaline phosphatase in the person's serum is determined.
What does alkaline phosphatase 207 do to body?
What causes the alkaline phosphatase to get to 158.
What are the isomers of alkaline phosphatase?
how many isoenzyme in alkaline phosphate
What is Lap score?
Leukocyte alkaline phosphatase
What is the principle of leukocyte alkaline phosphatase?
don't
Is Alkaline phosphatase 210 alarmingly high?
yes
What is alkaline phosphatase used for?
Alkaline Phosphatase has a number of uses within science and research. It is used to remove phosphate monoester. It is also used to remove phosphates from DNA.
What patient preparations are associated with alkaline phosphatase testing?
.A person being tested for alkaline phosphatase should not have anything to eat or drink, except water, for eight to ten hours before the test.
What level in the blood may be increased in osteomalacia and fracture healing?
Alkaline phosphatase is increased in osteoblastic bone tumors, rickets, osteomalacia, and during fracture healing. Levels can be determined through an alkaline phosphatase blood test.
Location of alkaline phosphatase in human?
Alkaline phosphatase is an enzyme found in most tissues of the body, but in medicine is generally used as a measure of LIVER health. Elevated ALP levels are often indicative of a bile duct obstruction.
What is the purpose of alkaline phosphatase testing?
Medical testing of alkaline phosphatase is concerned with the enzyme that is found in liver, bone,placenta, and intestine.When it is present in large amounts, it may signify bone or liver disease or a tumor.inflammatory bowel disease.
What blood analyte is affected by food intake?
glucose, iron, lipids and alkaline phosphatase
