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Q: Why is it important to select for transformed bacteria?
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Most of the insulin manufactured today is produced by .?

transformed bacteria


Antibiotic in gene cloning?

If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.


What is transformed from rough strain bacteria to smooth strain cells?

DNA


What atmospheric gas can only be used by plants when transformed by bacteria first?

Nitrogen


Explain how the bacteria in griffith's experiment were transformed?

The harmless living bacteria took in pneumonia-causing DNA(genes) from the heat-killed, pneumonia-causing bacteria, as a result of which the harmless bacteria changed into bacteria that cause pneumonia.


What is a blasticidin?

A blasticidin is any of a class of antibiotics used to select transformed cells in genetic engineering.


Human insulin is produced in large quantities by?

recombinant E. coli bacteria grown in large fermentation vats


Which gas makes up 78 percent of our atmosphere but can be used by plants only when transformed by bacteria?

nitrogen-


Who Concluded that bacteria could be transformed from harmless to disease by an unknown factor?

In 1927, Fredric Griffith concluded that bacteria could be transformed from harmless to disease-causing by introducing a factor that was not yet identified. This changing factor was eventually discovered to be DNA, the basis for all biological reproduction.


A plasmid a foreign gene and recombinant DNA would be associated with what type of organism?

A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).


What was 'transformed' in Griffith's experiment?

Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form.


Which gas makes up 78 percent of our atmosphere but can be used by plants only when transformed by bacteria first?

nitrogen-