Want this question answered?
DNA
recombinant E. coli bacteria grown in large fermentation vats
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
bacteria is important because inside their their body tissues, it provides them with nutrients.
Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.
transformed bacteria
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
DNA
Nitrogen
The harmless living bacteria took in pneumonia-causing DNA(genes) from the heat-killed, pneumonia-causing bacteria, as a result of which the harmless bacteria changed into bacteria that cause pneumonia.
A blasticidin is any of a class of antibiotics used to select transformed cells in genetic engineering.
recombinant E. coli bacteria grown in large fermentation vats
nitrogen-
In 1927, Fredric Griffith concluded that bacteria could be transformed from harmless to disease-causing by introducing a factor that was not yet identified. This changing factor was eventually discovered to be DNA, the basis for all biological reproduction.
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form.
nitrogen-