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It is important for someone who works with bacteria to have a genetically identical bacterial colony so they can test different things on one type of bacteria. Making observations on that one colony is valuable for visual research and identification of that microorganism.

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9y ago
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13y ago

It is important in microbiology to generate isolated colonies to help get a clear identification of the bacteria. If the colonies were not isolated they would become contaminated by other bacteria, and we would not be able to distinguish one from the other very easily.

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11y ago

this is done so that you can have a pure culture. and be able to identify the bacteria

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Q: Why is it important to start a bacterial culture with a single isolated colony?
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Why would you assume that an isolated colony is a pure colony of that microorganism?

Because bacterial cells reproduce and form identical daughter cells. Therefore, a single cell that is isolated away from the rest of the mixed bacterial culture will reproduce a colony of cells identical to itself. You will know that it is only that certain type of bacteria with none others mixed in, or pure :)


Why restreak isolated colony?

The isolated colonies for the new plate can be used to compare with the isolated colonies on the old plate. If the morphologies are the same, it can be assumed that pure culture is obtained.


What is a broth culture?

The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one


What does medical code 87086 stand for?

Pathology and Laboratory / Microbiology CPT code for: culture, bacterial; quantitive colony count, urine.


What is a broth?

The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one


Why can a single colony on a plate be used to start a culture?

The colony started from one single microbe that divided and divided until it formed what we call a colony of all the same bacteria. By selecting that colony we can be sure that its is pure and will grow only into a pure culture. Be sure to select on that is isolated so that you will not pick up a contaminate.


How an isolated colony forms?

Certain Isolation techniqiues are based on the concept that if an individual bacterial cell is separated from other cells and provided adequate space on a nutrient surface, it will grow into discrete mound of cells called a colony


Which colony was isolated?

be more specific, which colony of which sample from what experiment?


What is the circumference of the colony of bacterial colony of 10 mm?

3.140


Determine the population of bacteria based on the bacterial colonies?

Assume each colony started as a single bacteria in the original culture. Count the colonies you have and multiply up according to how diluted you made the culture and how much of the original culture you used.


Why colony represent a pure culture?

The colony started from one single microbe that divided and divided until it formed what we call a colony of all the same bacteria. By selecting that colony we can be sure that its is pure and will grow only into a pure culture. Be sure to select on that is isolated so that you will not pick up a contaminate.


What is broth culture?

The culture which contain an organism (bacterial colony) which you are required to grow in a broth media that is a media lacking solidifying agent agar. A pure culture should not contain other bacterial or fungal cells in it except the required or cultured one