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Robert Koch

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Q: Who hypothesized that a bacterial colony arises from a single bacterial cell?
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A certain bacterial colony originated from the division of a single bacterial cell Each cell in this colony will most likely?

synthesize the same proteins and enzymes


What is a pure colony in microbiology?

A colony that arises form a single mother cell. The colony should not touch another colony. A pure colony consists of 50-72 generations of cells arising from a single mother cell.


Why would you assume that an isolated colony is a pure colony of that microorganism?

Because bacterial cells reproduce and form identical daughter cells. Therefore, a single cell that is isolated away from the rest of the mixed bacterial culture will reproduce a colony of cells identical to itself. You will know that it is only that certain type of bacteria with none others mixed in, or pure :)


What is a bacterial culture containing a single species of organisms referred to as?

A bacterial culture containing a single species of organism is referred to as pure or axenic.


Does each discrete colony represent the growth of one cell on an agar plate?

This is one part of a two part answer. Q: Does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture? A: No because a discrete colony is the growth of the descendants of a single cell. The cell will self replicate it self on the growth medium to produce more copies of it self thus creating a colony. A single colony has many cells in the colony. Cells from the colony can be extracted and then placed in a pure culture for better growth. A.C.


What does a single bacterial cell divide into during binary fission?

Binary fission produces two identical bacterial cells.


Determine the population of bacteria based on the bacterial colonies?

Assume each colony started as a single bacteria in the original culture. Count the colonies you have and multiply up according to how diluted you made the culture and how much of the original culture you used.


Which metric unit when reporting a size of single bacterial cell?

micometers


Why is it important to start a bacterial culture with a single isolated colony?

It is important for someone who works with bacteria to have a genetically identical bacterial colony so they can test different things on one type of bacteria. Making observations on that one colony is valuable for visual research and identification of that microorganism.


Is a bacterial cell the same as a single celled organism?

No, not quite. A bacterium is a single-celled organism, for sure, but not all single-celled organisms are bacteria.


How you differentiate between Transformed bacterial colonies and satellite colonies?

Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.


What is the purpose of ethanol in the spread plate technique?

There are mainly two techniques in microbiology in bacterial cell inoculation. The first is when the colony is added to the plate and spread with a spreader across the entire plate in aseptic conditions. The second is called 16-streak and is used to isolate a single colony