DNA itself is made up of nucleotides. Nucleotides links with each other to form a DNA chain. In the process of DNA replication, parent DNA strand needs to be duplicated. Hence, to make a new strand of DNA it requires nucleotides.
DNA replication is must because It is necessary for the cell division to occur to double the genetic content to pass it to a daughter cell
It is necessary to add a nucleotide during DNA replication so that the leading strand elongates towards the replication fork.
RNA Primer
The hydrogen bonds that hold the two DNA strands together are broken. This creates two prongs and each are made up of a single strand of DNA that creates two new partners for the two strands (because they add nucleotides).
DNA polymerase 1,2,3 are enzymes involved in adding nucleotides during replication
DNA contains the map of how the cell runs and survives. Replication is needed when a cell has to replace itself or another cell. This provides a way for cells to renew itself. Each strand of DNA needs to be copied and added to the new cell that is being created. During replication, the nitrogenous bases separate and allow replication to happen. This the process. 1. Helicase is added to the DNA strand to separate the strands. It causes the nitrogenous bases to break apart and create a replication bubble. At the end of the bubble, there are replication forks that cause elongation of the DNA strands. Proteins prevent the strands from re-sticking. 2. DNA polymerase is used for process of adding new new nitrogenous bases to the separated strands. It starts at an origin of replication and moves in a direction from the 3' side of the strand to the 5' side. It can only add nucleotides in this direction. The strand that is synthesized continuously in this direction is known as the leading strand. 3. Replication moves in the same direction on both strands. It does not encounter much resistance on the leading strand which has the direction of 3' to 5'. The other strand has an anti-parallel direction to the leading strand it is complementary and runs in 5' to 3'. This is known as the lagging strand. This strand needs RNA primer to start the replication process. After the RNA primer starts the process, DNA polymerase starts adding the DNA nucleotides. This can only happen in small segments and is known as Okazaki segments. RNA primer is used for each segment to continue the replication process. After each segment is done, DNA polymerase removes the RNA primer segments and replaces them with DNA nucleotides. Ligase glues the different DNA segments together to create a continuous strand. 4. The end of the strands cannot be replicated and are left out of the replication. This area is referred to as telomeres and do not contain genetic material. The loss of this information does not change the expression of the genes. 5. During the synthesis, DNA polymerases scan the strands and makes sure that nucleotides are correctly matched. If there is an incorrectly matched nucleotide, enzymes fix the problem by replacing the nucleotide. Mutations within the DNA sequence are rare because of this proofreading. 6. After this process there are 2 complete strands of the same DNA. 7. If there is damage to DNA sequence it can be repaired through a process called nucleotide excision repair. Nuclease cuts out the incorrect matching and then replaces it with the correct DNA. Ligase then glues the strand back together. Damage can come from chemical or motor damage.
Blindly selecting from a bag filled with blue, green, red, and yellow beads *apex Kyah!
DNA polymerase adds bases to the 3' end during replication. It matches the c with G and A with U during replication. Never add to the 5' end!
DNA Polymerase is the enzyme which adds new nucleotides during replication.
DNA polymerases add nucleotides to the exposed base pairs according to base-pairing rules.
When the replication fork is moving towards DNA double strand in the direction 5'- 3', a "Single-strand Binding Protein" (or SSB) -a dnaB gene product- must be removed in order to allow DNA polymerase to add the following nucleotide.
why is it necessary to add ater to the staphylococcus but not the bacillus
The answer is: accurate replication of DNA
RNA Primer
The hydrogen bonds that hold the two DNA strands together are broken. This creates two prongs and each are made up of a single strand of DNA that creates two new partners for the two strands (because they add nucleotides).
An RNA primer.
to add complementary nucleotide respect to the old strand for new strand synthesis.....
Not necessary- you only add/exclude drivers
not really...but if you are bored and want to add extra facts about it and make sure you have a microscope cuz then how would u see them.