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Simple answer is that the sample is soluble in water.

The primary reason to use distilled water is to maintain the purity of the sample. Municipal water is treated with chlorine to prevent bacterial growth, plus the water picks up traces of metal from the pipes, valves and tanks it travels through on the way to your faucet.

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15y ago
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5d ago

Agar needs to be dissolved or rehydrated in freshly prepared distilled water to ensure that there are no impurities or contaminants present that could affect the growth of microorganisms in the agar medium. Using distilled water also helps prevent the introduction of unwanted bacteria or fungi that could compete with the intended microorganisms being cultured. Additionally, freshly prepared water helps maintain the proper pH and consistency of the agar medium for optimal growth conditions.

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12y ago

since agar medium is used for development of microbial colonies, freshly prepared distilled water is used to prevent contamination or formation of colonies of other unwanted microbes.

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Q: Why is it necessary to dissolve or rehydrate agar in freshly prepared distilled water?
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