0
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.
Wiki User
∙ 9y ago
Wiki User
∙ 11y agoMSA is a selective as well as a differential media.
Mannitol is the differential part, it indicates organisms that ferment mannitol. If mannitol fermentation is occurring, lactic acid will be produced, and the pH will drop causing the MSA plate to turn yellow. The salt portion is selective for halophiles; organisms that cannot withstand a high salt content will be unable to grow well.
Add your answer:
Earn +20 pts
Why can mannitol salt agar and EMB agar be described as both selective and differential media?
it is selective because only a salt tolerant can grow. 7.5 nacl it has diffrential properties also. if mannitol formentors turns yellow staphlococus aureus; but if it has no change then its a nonpathogenic staphlococci (s. edermis)
Can serratia marcescens grow in mannitol salt agar?
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
Why is Msa argar a selective medium?
Mannitol Salt Agar is selective for staphylococci as the high salt (sodium chloride) levels prohibit most other bacteria from surviving and it is differential as Staphylococci ferment mannitol, producing acid, lowering the pH and turning the media yellow. The development of yellow media presumes the bacteria to be pathogenic Staphylococcus (usually S. aureus). From A Photographic Atlas for the Microbiology Laboratory by Leboffe and Pierce.
What is isolation media in microbiology?
The growth media such as nutrient agar or Mckonkey agar is used to isolate microbial cells from the mix culture when inoculate on them. Some time speical media is used which selectivly grow some time of bacteria or a differential media which give different morphology of different bacterial species e.g. blood agar and Mannitol salt agar. It can be done by three ways: the spread plate method, the streak plate method, and the pour plate method.
Purpose of high salt concentration in the mannitol salt agar medium?
Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.
Why can mannitol salt agar and EMB agar be described as both selective and differential media?
it is selective because only a salt tolerant can grow. 7.5 nacl it has diffrential properties also. if mannitol formentors turns yellow staphlococus aureus; but if it has no change then its a nonpathogenic staphlococci (s. edermis)
Is Mannitol salt agar a selective or a differential medium?
Both
What reaction does the enzyme involved in Mannitol-salt agar test mediate?
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
Does bacillus subtilis ferment acid from mannitol salt agar?
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
Can serratia marcescens grow in mannitol salt agar?
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
What is mannitol salt test for and whats a positive test result?
Mannitol Salts agar is a selective and differential media used to identify Staphylococcus sp. The media is selective due to the high salt concentration which prevents all but halophiles from growing. The media is differential due to the presence of mannitol and the indicator Phenol Red. Staphylococcus aureus can ferment mannitol and produce lactic acid as a result. The media contains an indicator called Phenol Red which turns yellow in low pH environments. This results in a yellow halo around the S.aureus colonies. Staphylococcus epidermidis cannot ferment mannitol and so the colour of the media around these colonies does not change. Certain vibro species and other halophiles may grow on these plates some of these appear pink and some may be able to ferment mannitol leading to false positives. S.epidermidis is a normal commensal organism and grows on the skin. S.aureus is a potential pathogen, antibiotic strains exist and can be hospital accquired infections. Search for "MRSA" for more information.
What will grow on mannitol salt agar besides staph?
Mannitol Salt Agar (MSA) is a selective/differential media that inhibits the growth of gram negative bacteria. Only a few bacteria can tolerate the 7.5% NaCl found within this media. The high salt content selects for Staphylococcus and a few salt tolerant enterococci. The presence of the sugar mannitol gives this media its differential properties. When mannitol is fermented (utilized by the bacteria) microorganisms release acidic byproducts that change the pH of the surrounding media. The pH change is indicated by a change in the pH indicator (phenol red) from pink to yellow.
Why is Msa argar a selective medium?
Mannitol Salt Agar is selective for staphylococci as the high salt (sodium chloride) levels prohibit most other bacteria from surviving and it is differential as Staphylococci ferment mannitol, producing acid, lowering the pH and turning the media yellow. The development of yellow media presumes the bacteria to be pathogenic Staphylococcus (usually S. aureus). From A Photographic Atlas for the Microbiology Laboratory by Leboffe and Pierce.
HOW do mannitol salt hook up to glycolysis?
Mannitol is not a substrate for glycolisis
What ingredient makes mannitol salt selective?
the salt content
How do mannitol-salt agar tests work?
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
What two media include elevated concentrations of sodium chloride for preventing growth of most bacteria?
mannitol salt agar is one of them, not sure about the other one
