because methylene blue turns colourless when it is reduced by hydrogen. during respiration hydrogen is produced and instead of reducing NAD, it reduces methylene blue and turns methylene blue colourless. if methylene blue goes from blue to colourless then this shows that the cell is respiring as it is producing a suffiecient amount of hydrogen to decolourise methylene blue
Methylene blue is necessary for various applications, such as in medical diagnostics, staining biological tissues, and as a medication to treat methemoglobinemia. It is also used as a redox indicator in chemical reactions and as a dye in various industries. In microscopy, methylene blue can help highlight cell structures for better visualization.
When you added methylene blue to the living yeast cell, the methylene blue is actually a dye so you could see the cells, therefore, the yeast cell became blue. It is much the same with hair dye. If you have a bottle of blue hair dye, it will dye your hair blue.
Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.
Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable. Also used to staining the blood film and used in cytology. It gives a blue coloration to the human cheek cell.
When a tomato cell is stained with methylene blue, organelles such as the nucleus, vacuoles, and possibly some smaller structures like mitochondria or chloroplasts may become visible under the microscope. Methylene blue tends to bind to nucleic acids and can highlight these structures within the cell.
NADH
Methylene blue is necessary for various applications, such as in medical diagnostics, staining biological tissues, and as a medication to treat methemoglobinemia. It is also used as a redox indicator in chemical reactions and as a dye in various industries. In microscopy, methylene blue can help highlight cell structures for better visualization.
Methylene blue can inhibit the growth and reproduction of paramecium by disrupting cellular respiration and other metabolic processes. It interferes with the electron transport chain in mitochondria, affecting energy production in the cell and ultimately leading to its death.
When you added methylene blue to the living yeast cell, the methylene blue is actually a dye so you could see the cells, therefore, the yeast cell became blue. It is much the same with hair dye. If you have a bottle of blue hair dye, it will dye your hair blue.
Methylene blue is a solid, odorless, dark green powder at room temperature. In water this chemical compound turns into a blue solution. When methylene blue is reacted with yeast cells it inhibits the respiration which stops the cells from using hydrogen ions to release energy.
Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.
Methylene blue can act as a redox indicator by accepting or donating electrons in cellular processes. It may affect cell activity by interfering with electron transport chain function, disrupting mitochondrial activity, and potentially generating reactive oxygen species. Overall, its impact on cell activity depends on concentration, exposure duration, and specific cellular pathways.
Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable. Also used to staining the blood film and used in cytology. It gives a blue coloration to the human cheek cell.
NADH
NADH
When a tomato cell is stained with methylene blue, organelles such as the nucleus, vacuoles, and possibly some smaller structures like mitochondria or chloroplasts may become visible under the microscope. Methylene blue tends to bind to nucleic acids and can highlight these structures within the cell.
When staining a cheek cell with methylene blue, you can observe the cell's nucleus as a distinct blue color. The stain highlights the nucleus due to its affinity to bind with DNA. The rest of the cell may appear pale blue or colorless in comparison.