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Why is the E. coli lac Z gene used as a reporter gene in S. cerevisiae cells but not in E. coli cells?
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Describe how gene regulation occurs in bacterial cells?
Escherichi coli (E. coli) bacteria provide a good example of gene regulation - E. coli bacteria are genetically encoded to regulate production of enzymes that digest lactose only when lactose is present and no glucose is available.
Which technology allows strands of DNA to be transferred from the cells of one species into the cells of another species?
gene splicing
What is a reporter enzyme?
Reporter enzymes are enzymes that are covalently attached to an analyte in an immunoassay because of their ability to amplify a weak signal and increase detectability of this target molecule. They are generally used to report on antibody/antigen interactions. Reporter enzymes are used extensively in molecular biology because they have particular characteristics that allow visual, spectrophotometric detection of these immune complexes. E.g. HorseRadish Peroxidase is a widely used reporter enzyme, it produces a colour, fluorimetric or luminescent derivative of the labelled analyte allowing it to be detected and quantified.
Who invented gene therapy?
Gene Therapy is a gene delivery,the inserting genes (eg.via retrovirual vectors) into selected cells in the body in order to - cause those cells to produce specific theraputic agent. -cause those cells to become less susceptible toa conventional theraputic agent.
What does the gene ST2 responsible for?
The gene ST2 is responsible for the suppression of tumorigenicity. This means that this gene is responsible to suppressing tumors, or cells that may cause tumors.
What is deletion analysis in genetics?
5' deletion analysis is a method where upstream base pairs are systematically cut. for example -20 to +1 then the second would be -15 to +1 third -10 to +1 and so on. these segments are then bound with a reporter gene and transfected into E. coli cells. The E.coli cells are used to translate and transcribe the reporter genes. prepare the cell extracts and measure the reporter gene expression.
Describe how gene regulation occurs in bacterial cells?
Escherichi coli (E. coli) bacteria provide a good example of gene regulation - E. coli bacteria are genetically encoded to regulate production of enzymes that digest lactose only when lactose is present and no glucose is available.
When did Gene Sherman - reporter - die?
Gene Sherman - reporter - died in 1969.
When was Gene Sherman - reporter - born?
Gene Sherman - reporter - was born in 1915.
What are recombinant cytokines?
Recombinant cytokines, i.e., cytokines produced by expression from suitable cloning vectors containing the desired cytokine gene, can be expressed in yeast(see: Saccharomyces cerevisiae expression system), bacteria (see: Escherichia coli expression system), mammalian cells (see: BHK, CHO, COS, Namalwa), or insect cell systems (see: Baculovirus expression system). Answer given by P.Gangaraj,Sivakasi
What has the author Miles Trochesset written?
Miles Trochesset has written: 'Statistical learning of gene annotations in Saccharomyces cerevisiae'
A eukaryote used in genetic engineering is?
Virus and plasmid. Both can insert the target gene into the host's genome.
E coli makes insulin because?
the insulin gene was inserted into it. E. coli doesn't naturally produce insulin. It needs to be engineered to do so.
What is the lac operon in E. coli a classic example of?
gene expression occurring at transcription
Why do people get Adenomatous Polyposis Coli?
APC is usually associated with inheritance of a faulty gene from a parent. The APC gene is required to stop cells growing too fast: knocking out the other copy of the gene in the body (by chemical damage or random DNA damage) removes this 'brake' and allows lots of little polyps to form in the intestine.
Which is an advantage of using E. coli to make a human gene project?
its genes are well known
What has the author Michael Butlin written?
Michael Butlin has written: 'An investigation of the DNA sequences required for the transcription ofthe ribosomal RNA gene of Saccharomyces cerevisiae'
