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What is a reporter enzyme?

Updated: 8/11/2023
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15y ago

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Reporter enzymes are enzymes that are covalently attached to an analyte in an immunoassay because of their ability to amplify a weak signal and increase detectability of this target molecule. They are generally used to report on antibody/antigen interactions. Reporter enzymes are used extensively in molecular Biology because they have particular characteristics that allow visual, spectrophotometric detection of these immune complexes. E.g. HorseRadish Peroxidase is a widely used reporter enzyme, it produces a colour, fluorimetric or luminescent derivative of the labelled analyte allowing it to be detected and quantified.

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15y ago
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9y ago

Reporter genes are genes that produce a product easily detected in eukaryotic cells. It is used as a marker to determine the activity of another gene with which its DNA has been closely linked or combined.

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15y ago

a gene that researchers attach to another gene of interest in cell culture, animals or plants http://en.wikipedia.org/wiki/Reporter_gene

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A reporter enzyme monitors transformation of host cells by foreign DNA in addition to a selectable marker through insertional inactivation. It differentiates recombinants from non recombinants.


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The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity


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What to enzymes do in an enzyme-substrate?

in an enzyme-substrate complex, the enzyme acts on the substrate .


How a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?

reporter enzymes can differentiate recombinants from non- recombinants on the basis of their ability to produce specific color in the presence of a chromogenic substrate.[ ref. insertional inactivation of beta-galactosidase when rDNA is inserted and formation of colored and non colored colonies accordingly].


Does enzyme has proteins?

An enzyme is a protein


What is the active site for an enzyme?

The place where the substrate and the enzyme meet to allow the enzyme to function.


What is an angiotensin converting enzyme?

An angiotensin converting enzyme is an enzyme which catalyzes the creation of angiotensin.


What enzyme is responsible for fat digestion?

the lipase enzyme :)


The binding together of an enzyme and a substrate forms a what?

enzyme-substrate complex


An enzyme that cuts double-stranded DNA at specific nucleotide sequences?

Such an enzyme is called a restriction endonuclease


What is it called when and enzyme and substrate come together?

The binding of an enzyme and a substrate forms an enzyme-substrate complex. It lowers the activation energy of a chemical reaction