Want this question answered?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
lower, solvent, lower, solvent
If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed. These photos show how the yellow compound is running into the solvent when lifted from the developing jar.
Collisions between solvent molecules and solute are more frequent at higher than lower temperatures.
Sea level has been higher and lower than it is currently.
If the spots are submerged in the solvent, they are washed off the plate and lost.
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
lower, solvent, lower, solvent
Most solutions have a HIGHER boiling point than the pure solvent. A solution with a lower boiling point than the solvent has formed an azeotrope.
If the colored spot is below the level of the solvent, the spot will simply dissolve into the solvent.Chromatography works because the solvent climbs up the 'column' by capillary action, picking up the colored spots along the way and dragging them along. Because of the chemistry of the colors and the solvents, some of the colors in the spots are easier to drag along the column than others, and move along faster.
If the solvent level in the developing jar is deeper than the origin (spotting line) of the TLC plate, the solvent will dissolve the compounds into the solvent reservoir instead of allowing them to move up the plate by capillary action. Thus, you will not see spots after the plate is developed. These photos show how the yellow compound is running into the solvent when lifted from the developing jar.
more
Collisions between solvent molecules and solute are more frequent at higher than lower temperatures.
The lower level is .01, which is much lower than .08.
A lower than normal level of oxygen is called hypoxia.
Sea level has been higher and lower than it is currently.
air-conditions are usually placed at a higher level rather than at a lower level.