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Why is the media inoculated with the swab before the swab from a patient specimen is used to make a smear?
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What type of media should be inoculated using zig-zag motion across the surface of the media?
agar slant
Why does bacteria on agar plates need to be removed from the incubator after 24-48 hours?
To avoid overgrowth of the bacteria you have inoculated. Depending on the type of media you use, you could also have a false reading if it is left in there for the wrong amount of time.
Was life different before mass media?
Life certainly was different before mass media. Life was much more simple and less about how a person looked or what they did.
What type of media is used for growing a culture from a throat specimen?
blood and chocolate agar plates as well as in universities laboratory Nutrient agar plates are also provided
What is chemical composition of tributyrin agar media?
I have never used this before; however, it seems you add 10g of tributyrin per liter of nutrient media for fungi.
What type of media should be inoculated using zig-zag motion across the surface of the media?
agar slant
What are Mushroom Logs or Compost?
Mushroom Logs/Composts contains growing media/substrate inoculated with mushroom spawn. The white/brown material inside the bags is the "mycelium", which has "colonized" the growing media/substrate.
How do you inoculate a solid media?
To inoculate a solid media, a sterilized inoculation loop or needle is used to pick up a small amount of the desired culture. This culture is then streaked onto the surface of the solid media in a specific pattern to ensure isolated colonies grow. The inoculated media is then incubated at the appropriate temperature to allow the colonies to grow.
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
Why should you use an inoculating needle when making smears form solid media?
You should use an inoculation needle for making smears from a solid media so you can control how much specimen is put onto a slide. This method is easier for solid media and you would use a loop for liquid media.
Why does bacteria on agar plates need to be removed from the incubator after 24-48 hours?
To avoid overgrowth of the bacteria you have inoculated. Depending on the type of media you use, you could also have a false reading if it is left in there for the wrong amount of time.
When is irrigation for impacted cerumen contraindicated?
Irrigation should not be used to remove cerumen if the patient's eardrum is ruptured or missing; if the patient has a history of chronic otitis media.
In microbiology a specimen came from a patient with an unknown infection what test might you perform to help physician to effectively treat the patient?
Conventionally, the first "test" performed on many specimens arriving in the clinical microbiology laboratory is a direct microscopic examination. This typically involves staining a smear of the specimen on a microscope slide by the Gram's staining method, an Acid-Fast staining method, or, if a fungal infection is suspected, a technique that allows for visualization of fungal elements within the specimen (e.g., Calcofluor white stain). Additionally, more specific stains can be performed depending upon the etiologic agent suspected. For example, fluorescent antibody "stains" that are specific for certain respiratory viruses can be used to directly probe the specimen for said virus(es).The next step of the process is usually growing the microorganism(s), which allows for identification and, subsequently, antimicrobial drug sensitivity testing. Traditionally, cultures of bacteria and fungi are grown in either Petri dishes and/or in glass tubes containing growth medium that supports the growth of either a wide variety of microbes (supportive or non-selective media) or only a few specific types of microbes (selective media). After inoculation and incubation of the media, microbial growth can be identified by many different means, including more advanced molecular methods. Finally, antimicrobial drug sensitivity testing will be performed on the growth, if such testing is appropriate. From this information, the physician will be able to decide upon one or more antimicrobial drugs that are useful for treating the infection.Viruses, however, require different types of testing. Specimens for virus identification are typically inoculated onto a growing monolayer of mammalian cells (e.g., human newborn foreskin cells, monkey kidney cells, etc.). Since viruses require a living cell in which to replicate, specimens for virus culture cannot be inoculated onto an artificial medium such as blood agar, for example. Subsequently, the cell monolayers are incubated and periodically examined for evidence of replication of viruses. In some instances, viruses will destroy or alter the cells in which they've replicated; this is evidenced by sometimes virus-specific cytopathic effect (e.g., rounding of cells, complete obliteration of some areas of the cell monolayer, syncytia formation, etc.). In other instances, there's very little evidence that the virus has infected and replicated within the cells of the monolayer. Regardless, samples of the cells are transferred to microscope slides and probed with virus-specific antibodies conjugated to fluorescent molecules. When viewed under a fluorescent microscope, cells will fluoresce if infected with a particular virus after staining with the virus-specific antiserum. Also, viruses can be detected from the cell cultures, and even the original patient specimen, using molecular techniques, such as polymerase chain reaction (a.k.a. PCR). After identification of the virus or viruses infecting the patient, a physician can choose an appropriate anti-viral drug that combats the infection, if one is available.For eukaryotic parasites (e.g., tapeworms, malaria, Giardia), sometimes the only means for identification will be direct microscopic exam of the specimen, as culturing these critters is very time consuming, requires very specialized media, or is sometimes impossible. Also, serologic techniques are useful for identification of some parasites.I'm hopeful that this information is helpful to you. Good luck!- Microbioman
Who owns the information contained within medical records?
The patient owns the information; the doctor owns the media or paper.
Was life different before mass media?
Life certainly was different before mass media. Life was much more simple and less about how a person looked or what they did.
Did social media begin before the Internet was public?
Nope.... social media grew out of the expansion of the internet.
What has the author S Javadian written?
S. Javadian has written: 'A multi-media patient management system for glaucoma patients'
