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How can you further isolate pigments on a thin layer plate in TLC experiment?
Run the mixture on the TLC plate. Find the different colored pigments. Scrape each spot and elute the pigments.
Why iodine is used in thin layer chromatography?
The staining of a TLC plate with iodine vapor is among the oldest methods for the visualization of organic compounds. It is based upon the observation that iodine has a high affinity for both unsaturated and aromatic compounds. Preparation: A chamber may be assembled as follows: To 100 mL wide mouth jar (with cap) is added a piece of filter paper and few crystals of iodine. Iodine has a high vapor pressure for a solid and the chamber will rapidly become saturated with iodine vapor. Insert your TLC plate and allow it to remain within the chamber until it develops a light brown color over the entire plate. Commonly, if your compound has an affinity for iodine, it will appear as a dark brown spot on a lighter brown background. Carefully remove the TLC plate at this point and gently circle the spots with a dull pencil. The iodine will not remain on the TLC plate for long periods of time so circling these spots is necessary if one wishes to refer to these TLC's at a later date.
Is a TLC Plate the same as filter paper?
No. A TLC (Thin Liquid Chromatagraphy) plate is made specially. It has different Compounds to it that make it separate from filter paper. See 1st related link below for more info on TLC Plates See 2nd related link for info about filter paper
Why is that the centre of the spot is considered when calculating Rf values for TLC?
outside are jus impurities
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
What is tailing effect in TLC?
Tailing effect is an error that occurs when the spot is not allowed to dry hence leading increased diameter of the spot in a TLC plate. Nwando Ata.
How can you further isolate pigments on a thin layer plate in TLC experiment?
Run the mixture on the TLC plate. Find the different colored pigments. Scrape each spot and elute the pigments.
What is the solvent front and how is its migration on the TLC plate measured?
The solvent front is the line at where the solvent moves up the TLC plate. Its migration can be measured by taking the R value of the TLC plate.
What problem will ensue if the level of developing liquid is higher than the applied spot in a TLC analysis?
If the spots are submerged in the solvent, they are washed off the plate and lost.
When preparing to develop a TLC plate what is the appropriate spatial relationship between the level of solvent in the developing chamber and the spots on your TLC plate?
The spots on the TLC plate should be placed about a cm from the bottom of the plate. Then solvent should then fall about half-way between these spots and the bottom of the beaker.
Why can the pigment spot on chromatography paper not be submerged in solvent?
This is because the nonpolar pigments would just simply dissolve in the nonpolar solvent instead of traveling up the TLC plate.
Why iodine is used in thin layer chromatography?
The staining of a TLC plate with iodine vapor is among the oldest methods for the visualization of organic compounds. It is based upon the observation that iodine has a high affinity for both unsaturated and aromatic compounds. Preparation: A chamber may be assembled as follows: To 100 mL wide mouth jar (with cap) is added a piece of filter paper and few crystals of iodine. Iodine has a high vapor pressure for a solid and the chamber will rapidly become saturated with iodine vapor. Insert your TLC plate and allow it to remain within the chamber until it develops a light brown color over the entire plate. Commonly, if your compound has an affinity for iodine, it will appear as a dark brown spot on a lighter brown background. Carefully remove the TLC plate at this point and gently circle the spots with a dull pencil. The iodine will not remain on the TLC plate for long periods of time so circling these spots is necessary if one wishes to refer to these TLC's at a later date.
What is the component of the coating on the pre-coated TLC plate?
silica or alumina
Why KMnO4 used in TLC?
because it can make the point shown in your plate
Is a TLC Plate the same as filter paper?
No. A TLC (Thin Liquid Chromatagraphy) plate is made specially. It has different Compounds to it that make it separate from filter paper. See 1st related link below for more info on TLC Plates See 2nd related link for info about filter paper
Why is that the centre of the spot is considered when calculating Rf values for TLC?
outside are jus impurities
How do you rent a car with a TLC license plate?
You have to be speaking Bangalian,Indian or a good Spanish.
