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To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.

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Q: Why loop and cap should not be laid on workbench while transferring the inoculum?
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Why loop and cap should not be laid on workbench while transferring inoculum?

To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.


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