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To denature the DNA

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Q: Why must DNA fragments be exposed to an alkaline solution before hybridization?
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Related questions

What is the color of pH strip when the water has a 9.5 pH level?

First of all it is NOT water at this pH level. It is an ALKALINE Solution. With a pH = 9.5 the colourt would be blue.


Why does phenolphthalein change color in alkaline pH?

Phenolphthalein is a compound with chemical formula of C20 H14 O4. It is colorless but turns pink in alkaline solutions. This is because it undergoes a process of ionization where positively or negatively charged ions are added or removed from a molecule, which converts the molecule into ions. Phenolphthalein molecule contains positively charged hydrogen ions and is naturally colorless with bright pink ions. When exposed to an alkaline solution, these hydrogen ions are removed, phenolphthalein molecule gets ionized and the solution turns pink. The stronger is the alkali solution, more hydrogen ions removed and stronger pink colour of the phenolphthalein solution.


Turns blue litmus paper to red?

Bases turn red litmus paper blue. Alkali (ammonia) Alkaline


How are Alkaline metals compared to alkali metals?

alkali metals are the "mad" elements who explode when exposed to a certain element. for example, sodium cannot be exposed to hydrogen, or it will explode. on the other hand, alkaline earth metals are good conductors, but they do not explode. they also come only from the earth and its crust.


Why does crushing solute increases the rate of solution?

More surface area is exposed.


What changes do you expect to see when the cells are exposed to the solution?

trying to figure this out 2!!


Is scoria volcanic glass?

Yes.Scoria ("lava rock") is found on the surface of lava flows or in fragments that have ejected from the volcano's explosive opening. In these conditions, the molten rock was immediately exposed to the open air on the Earth's surface, causing it to cool and solidify very rapidly. This solidification process is too quick for atoms within the solution to bind together to form large crystals, so the solid rock is consequently made up of tiny glassy fragments that are amorphous, or do not contain crystalline structures. Since scoria is made up of these glass fragments, it is defined as VOLCANIC GLASS.


What metal could explode if you reacted it with an acid?

All the alkaline metals will explode violently when exposed to H2O. The further down you go on the Periodic Table of the elements, the more reactive they become.(Hope this helps!)


What are low-order detonations?

they leave large ordnance fragments containing explosives and may leave chunks or pieces of exposed explosives; do not remove remaining debris.


What do low order detonations do?

leave large ordinance fragments containing explosives and may leave chunks or pieces of exposed explosive, do not remove remaining debris


What happens in low order detonations?

leave large ordance fragments containing explosives and my leave chunks or pieces of exposed explosive do not move remaining debris


What happens to vitamin c when exposed to air?

The concentration of vitamin C in the solution decreases.