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During the experiments for genetically engineered plasmids, a large number of cells are used because the frequency of insertion and recombination of the target gene is very low. This also generates a large number of cells in which the plasmid may not be taken up at all. In order to differentiate genetically engineered cells from normal ones, genetic markers are used which quite frequently are related to some physiological effect.
What else can I help you with?
Why must a genetically engineered plasmid contain a genetic marker?
During the experiments for genetically engineered plasmids, a large number of cells are used because the frequency of insertion and recombination of the target gene is very low. This also generates a large number of cells in which the plasmid may not be taken up at all. In order to differentiate genetically engineered cells from normal ones, genetic markers are used which quite frequently are related to some physiological effect.
A gene that makes it possible to distinguish bacteria that carry the plasmid from those that dont?
Genetic marker.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
What is the Difference between physical marker and genetic marker?
Physical markers are easily observable traits like eye color, while genetic markers are specific sequences in the DNA that are associated with a particular trait or disease. Physical markers can be seen directly, while genetic markers require testing to identify.
What gene makes it possible to identify bacteria that carry a plasmid?
The gene commonly used to identify bacteria carrying a plasmid is the beta-lactamase gene, which confers resistance to beta-lactam antibiotics. Bacteria harboring plasmids with this gene can be identified by growing them on agar plates containing beta-lactam antibiotics and observing which colonies survive.
What would happen if you cut both the jellyfish glo gene and puc18 plasmid with the ecor1 restriction enzyme?
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?
Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.
What can gene for antibiotic resistance be used for?
genetic marker
How can one detect polymorphism by genetic marker?
One can detect polymorphism by genetic marker using single-nucleotide polymorphism which is able to even tell mutation of a gene.
What does a marker contain?
Markers contain ink in the inside. Like pens they contain ink.
What is the purpose of the ampicillin in the transformation procedure?
The ampicillin resistance gene (AmpR) is called a selectable marker gene and is incorporated into several plasmids that are commonly used in a wide range of genetic engineering and molecular biology experiments. The function of a selectable marker gene is to provide the host containing the plasmid a certain property that is not inherently present in the host. For example, the AmpR gene codes for a protein that makes cells containing this gene resistant to the antibiotic ampicillin. Therefore, when plasmids are successfully transfected into bacterilal cultures, one can assess which colonies have taken up the plasmid by looking at which ones survive onampicillin-containing media. Those that do not survive do not have the plasmid. The surviving colonies can then be picked up and carried on to the next stage of experimental research.
What are plasmids and how can they be used to create transgenic organisms?
A plasmid is circular piece of DNA that contains all the genetic information that allows it to bepropagatedinside a living organism. Plasmids are host specific, therefore, a plasmid used in E. coli won't work in yeast. There may be some exceptions.A plasmid will contain some of these genetic elements.1. ) Origin of replication - The replication machinery used by the host is compatible with replicating the plasmid as well.2.) Selection marker - A gene that codes for an enzyme or a protein that givesresistanceto an antibiotic of choice. Kanamycin, ampicillin etc.3.) MCS - Multi cloning site - DNA sequences which have been specifically designed to contain sites for nucleases so that a gene can be inserted in the right place with the right orientation and transcription frame.4.) Expression elements - Basically promoters, terminators, ribosome binding sites etc.If you insert a piece of DNA that the organism didn't have before, it gives that organism some new properties. For example, if the gene codes for a protein that makes it resistant to a number of antibiotics then now you have a new transgenic organism.That is a poor way of doing it though. More sophisticated way would be to have DNA recombination sites on the plasmid that are homologous with DNA recombination sites on the organism's genome so that the plasmid can beincorporatedinto the genome.
