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Why must the inoculating loop be cooled before touching a culture?
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∙ 10y ago
To avoid killing the test subjects before the test can begin.
Joany Funk
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What are the other inoculating instruments aside from needle?
Aside from the inoculating needle, an inoculation loop, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. Its tip is a wire made of platinum or nichrome, the latter being inferior but less expensive. The wire forms a small loop with a diameter of about 5 mm. This loop is handy for taking an inoculum from a liquid by using the phenomenon of surface tension. It is also called a smear loop, inoculation wand or microstreaker, The loop is used to cultivate microbes in Agar jelly and to use the streak maneuver to transfer microbes. The inoculation loop is always sterilized in a flame until it becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. After flame sterilization, the loop must be cooled so that the next cells to touch the loop don't instantly die.
What are the differences between streak plate technique and pour plate technique?
Streak Plate:Pure colonies of bacterial or other microorganisms can be obtained on petri dishes by streak plate. The microbial mixture is transfered to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in one of several patterns. After the first sector is streaked in dish, the inoculating loop is sterlized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cells will drop from it as it is rubbed along the agar surface. These develop into seprate colonies.Pour Plate:Extensively used with procaryotes and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. Then small volumes of several diluted samples are mixed with liquid agar that has been cooled to about 45oC and the mixture are poured immediately into sterile culture dishes. Most bacteria and fungi are not killed by a brief exposure to the warm agar. After the agar has hardened each cell is fixed in place and forms an individual colony.
Why seeds can germinate by using tap water and seed cant germinate by using boiled water cooled to room temperature?
Tap water also contains some ammount of oxygen in dissolved form which is needed by the seed during germination for respiration. in boiled and cooled water oxygen is removed during boiling. Hence seed can germinate in tap water and not in boiled and cooled water.
How do you prepare a pure culture from a mixed broth culture?
There will never be a pure culture in this system of things. However, depending on what you believe in of course, there is to come a time when all the disturbing mixed up things we deaql with on a daily basis will come to pass and a new earth will form. It will be at that time when we can truly obtain a pure culture. Just my opinion, take it for what it is, but know I'm basing it on over 30 years of theocratic study. Have a blessed day. ^ This person is a retard, it's a microbiology question, they aren't referring to a culture of people, they are referring to a culture of microbes. And it took me 2 seconds to look up the real answer. So: A mixed culture is a mix of different strains of an organism / bacteria. A pure culture is a culture consisting of only one strain. YOU CAN OBTAIN A PURE CULTURE BY PICKING OUT A SMALL PORTION OF THE MIXED CULTURE(which will form the pure culture) AND GROWING THEM ON A NEW CULTURE MEDIA. It's like taking 2 animals out of a herd and breeding them, and breeding only within their family, so they all look exactly the same. Except bacteria can reproduce asexually so you just need one.
What will result when 1 to 5 agar is added to nutrient broth boiled and cooled?
Solid medium
Why must the inoculating loop be cooled first before touching a culture?
To avoid killing the test subjects before the test can begin.
Why should you let the inoculating loop cool first before and after using it?
The inoculating tube is cooled before use so that the organism is not killed by extensive heat.
Why cooling the inoculating instrument prior to obtaining the inoculum?
The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.
Why is the loop allowed to cool before putting it into the bacterial culture?
Because too much heat can kill bacteria. Think of pasteurised milk - the pasteurising process involves rapid heating and cooling to kill off any bacteria that might be present in the milk.
Why should nutrient agar be cooled to 50 c before inoculating the bacteria?
To minimise condensation on the lid of the Petri dish.
Why is culture medium cooled 48 temperature before is poured?
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
What are the other inoculating instruments aside from needle?
Aside from the inoculating needle, an inoculation loop, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. Its tip is a wire made of platinum or nichrome, the latter being inferior but less expensive. The wire forms a small loop with a diameter of about 5 mm. This loop is handy for taking an inoculum from a liquid by using the phenomenon of surface tension. It is also called a smear loop, inoculation wand or microstreaker, The loop is used to cultivate microbes in Agar jelly and to use the streak maneuver to transfer microbes. The inoculation loop is always sterilized in a flame until it becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. After flame sterilization, the loop must be cooled so that the next cells to touch the loop don't instantly die.
Why is the milk cooled before the bacteria are added?
Because it is. face it
What happened to water vapor before it turns to water?
It cooled.
Why is tar heated and not cooled before applied to a roof?
Tar is heated so it can stick to what ever it is going on. If it was cooled it would not stick right.
Why spices are cooled before blending?
cause that makes it taste better
Why are the acidic extracts and the basic extracts cooled before neutralization?
jjgg
