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Why cooling the inoculating instrument prior to obtaining the inoculum?
Wiki User
∙ 9y ago
The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.
Wiki User
∙ 9y ago
Wiki User
∙ 9y agoThe inoculating instrument must be cooled first before obtaining the inoculum. This prevents the bacteria from getting fried. Dead bacteria do not grow nor produce cultures.
Wiki User
∙ 13y agootherwise the heat would kill the bacteria (inoculum)
Wiki User
∙ 12y agoIf the inoculating instrument is too hot when obtaining the inoculum, it will "fry" the organism and kill it. It will, then, not grow when it is cultured.
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