You might crack the glass.
They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.
They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.
The basic requirement for you to see an object using the microscope is light. The amount of light will determine the visibility of the specimen.
Samples for observation by an electron microscope are typically prepared by fixing the specimen in a chemical solution, dehydrating it, and then embedding it in a resin for stability. The sample is then sliced into ultrathin sections using a microtome before being stained to enhance contrast. Finally, the prepared sample is placed inside the electron microscope for imaging.
Having the underside of the microscope slide dry prevents water droplets from interfering with the light passing through the slide, which could result in distorted or unclear images when viewed under the microscope. Additionally, a dry slide ensures that the specimen is in direct contact with the glass surface, facilitating better observation and imaging.
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
The lowest power objective lens should be in place when placing a slide on a microscope stage. This allows for easier focusing and initial viewing of the specimen at a lower magnification before switching to higher magnifications.
The light must be off
Electron Microscope
Drying the slide before placing it on the microscope stage helps prevent water droplets from distorting the sample or affecting the quality of the image. Water can create a barrier between the objective lens and the sample, leading to poor resolution and focus. Additionally, drying the slide helps prevent the growth of microorganisms that may otherwise thrive in a moist environment.
Two types of microscope use two types of illumination. The old reflect mirror type use a mirror beneath the slide to gather light from the room and concentrate it on the slide. You wiggle the mirror around while looking through the 'scope until you get the brightest field. The second type of microscope uses an integral light source located beneath the slide. These are expensive, because the light must be as pure as possible and evenly bright across the viewing field.
The objective must be in the upper position when removing or placing a new slide on the stage of your microscope. This prevents the potential for the lens to hit the slide and possibly damage both the lens and the slide.