Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
size and shape
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
Gram negative bacteria are typically rod-shaped (bacilli) or corkscrew-shaped (spirochetes). They have a double cell membrane composed of an inner and outer membrane, which contributes to their staining properties in the Gram staining technique.
Negative staining is also known as indirect staining because the stain does not directly interact with the specimen.
The classification of cyanobacteria is based on Gram staining, which is typically negative.
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
size and shape
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
The acid-fast staining result for the sample is positive.
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
Only if it is dried up & staining on your red dress.
Bacteria .
Air-drying smears during bacterial staining is essential to fix the cells to the slide. This helps prevent cell loss when they are subsequently stained and washed in the staining process. Air-drying also helps to preserve the cellular morphology and structure for accurate microscopic examination.
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
Those which show pink colour in gram staining are gram negative bacterialike E.coli