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What else can I help you with?
Why you perform thin and thick blood smear?
Smears are prepared to study microscopic features of a specimen. If we use thisk smears, then it would be difficult to study morphologic features. That's why, thin smears are preferred over thick smears
Three factors that may affect gram stain reaction?
(i) The age of bacterial culture should not be more than 24 h. At older age cell loses Gram positivity and will appear as Gram negative. (ii) Application of heat during the fixation of smears is another important step. Too much heating during this step will lead to loss in, Gram positiveness. (iii) Overcrowding of cells in smear also affects the result, due to improper decolourization. (iv) Staining reagents should be freshly prepared. (v) In Gram staining decolourizing step is very important. To obtain satisfactory differentiation, the nature and the exposure time of decolourizing agent should be standardized with the material to be stained. Acetone alone is more powerful decolourizing agent than ethanol. (vi) It is also important not to allow a .bacterial smear to dry. There are many variations of original Gram staining procedure
Why are thick or dense smears less likey to provide a good smear preparation for microscopic evaluation?
Thick or dense smears contain too many cells that can overlap, making it difficult to see individual cells clearly under the microscope. This can lead to inaccurate interpretation of the sample. A thinner smear allows for better visualization of individual cells and structures.
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
What is the quality control of blood smear?
The quality control of blood smears involves checking for proper staining, appropriate cell distribution, presence of artifacts, and the overall morphology of red blood cells, white blood cells, and platelets. Quality control also includes ensuring that the sample is adequately spread and covers the entire slide without clumping or overlapping of cells. Regularly monitoring and reviewing blood smears can help ensure accurate and reliable results for diagnosis and interpretation.
Bacterial smears are fixed before staining to?
Bacterial smears are fixed before staining to adhere the bacteria to the slide and kill the bacteria, which helps to prevent them from washing away during the staining process. Additionally, fixing the bacteria helps to preserve their cellular structures and prevent distortion during staining.
Explain why the platinum wire is repeatedly flame-sterilized in the preparation of bacterial smears?
To avoid any contamination.
Why is it essential that the smears initially be air-dried?
if not do this and fix it , it will be wash off when stain it.
Why it is essential that smears air-dried before heating?
Air-drying smears before heating is essential because it allows the cells to adhere firmly to the slide, preventing them from coming off during the heating process. This ensures that the cells are evenly distributed and fixed to the slide, resulting in a clear and accurate staining. Additionally, air-drying helps to remove excess water from the sample, which can interfere with the staining process.
When observing the prepared bacterial smears at the 1000X magnification should your iris diaphragm be all the way open or stopped down for minimal light?
The iris diaphragm should be partially closed to allow for optimal contrast when observing bacterial smears at 1000X magnification. This setting helps improve visibility of the specimen by reducing glare and enhancing details in the sample.
What is the function of a Bunsen burner in microbiology?
In microbiology, a Bunsen burner is used to sterilize tools and heat-fix bacterial smears onto slides for staining purposes. The flame produced by the Bunsen burner provides a sterile environment to prevent contamination during microbiological procedures.
What is the discussion of bacteria smear?
A bacteria smear refers to a technique wherein a small sample of bacteria is spread on a microscope slide for observation. The discussion surrounding bacteria smears typically revolves around examining the morphology, arrangement, and staining characteristics of the bacteria. It is an essential tool for identifying and studying bacterial species.
When was Jammy Smears created?
Jammy Smears was created in 1976.
Are pap smears painful after you get older?
Yes they are. The last 2 I have had have been very painful. I am 57 years old.
What is the legal age to start getting pap smears?
There is no law that determines the time to start pap smears. Pap smears are recommended for women 21 to 65.
Are fixed smears of specimens required in order to perform the Gram stain and endospore stain on the specimen?
Yes, fixed smears of specimens are required to perform both the Gram stain and endospore stain. Fixing the smear allows the cells to adhere to the slide, preventing them from washing away during the staining process. Additionally, fixation helps preserve the cellular structure, which is essential for accurate staining and observation of the bacteria's characteristics.
What particular activity that you can use alcohol lamp?
Alcohol lamps are commonly used in scientific laboratories for tasks such as heat-fixing bacterial smears, sterilizing inoculating loops, and providing a flame source for small experiments.
