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(i) The age of bacterial culture should not be more than 24 h.
At older age cell loses Gram positivity and will appear as Gram negative.
(ii) Application of heat during the fixation of smears is another important step. Too much heating during this step will lead to loss in, Gram positiveness.
(iii) Overcrowding of cells in smear also affects the result, due to improper decolourization.
(iv) Staining reagents should be freshly prepared.
(v) In Gram staining decolourizing step is very important. To obtain satisfactory differentiation, the nature and the exposure time of decolourizing agent should be standardized with the material to be stained. Acetone alone is more powerful decolourizing agent than ethanol.
(vi) It is also important not to allow a .bacterial smear to dry.
There are many variations of original Gram staining procedure
What else can I help you with?
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What is the yellow stain in my husband's underwear?
The yellow stain in your husband's underwear could be caused by a variety of factors, including sweat, urine, or residual body oils. It could also be a result of not properly rinsing out laundry detergent during washing. If the stain persists, consider using a stain remover or soaking the underwear before washing.
What is the difference between Jensen's version ang hucker's modification in gram staining based on reagents and gram reaction to fungi?
What is modified in Jensen's modification of Gram stain? Jensen's modification: This method involves use to methyl violet as primary stain, iodine and potassium iodide in water as mordant, absolute alcohol as decolorizer and neutral red as counterstain
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
What is schmorl's stain principle?
Schmorl's stain is a histological staining technique used to differentiate between different types of tissues in a sample based on their affinity for the stain. It is commonly used in pathology to help visualize specific cellular structures or abnormalities.
What factors can affect the final stain color of wood?
The final stain color of wood can be affected by factors such as the type of wood, the type of stain used, the application method, the wood's natural color, and the presence of any previous finishes or treatments on the wood.
Why won't wood take stain properly?
Wood may not take stain properly due to factors such as uneven absorption, previous finishes or treatments, or the type of wood being used. These factors can affect how the wood interacts with the stain, leading to inconsistent or blotchy results.
Can you tell how old a blood stain is?
No, the age of a blood stain cannot be determined solely by looking at it. Factors like environment, fabric type, and blood volume can affect the appearance of a stain. In forensic investigations, chemical testing and analysis are used to estimate the age of a blood stain.
What three types of data are obtained from the gram stain?
The three types of data obtained from a Gram stain are cell morphology (shape and size of the cells), cell arrangement (how cells are grouped together), and Gram reaction (whether cells are Gram-positive or Gram-negative based on their cell wall composition).
Is bleaching a stain endothermic or exothermic reaction?
Bleaching a stain is typically an exothermic reaction, meaning it releases heat to the surroundings. The chemical reactions involved in bleaching stains usually generate heat as they break down the stain molecules, resulting in a warming effect.
Why is the stain still tacky after a week?
The stain may still be tacky after a week due to factors such as high humidity, improper application, or using a low-quality stain.
What is a three letter word for stain?
dye
Anyone have an allergic reaction to using Cabot Australian Timber Oil stain?
Just stained s door, and no reaction at all.
How do rags with oil based wood stain spontaneously catch fire?
the stain and the rag create a chemical reaction that causes it to rot and decompose very quiclky. this chemical reaction creates heat, and if it gets hot eanough, fire.
Does pH influence the gram stain reaction?
pH does not directly influence the Gram stain reaction. The Gram stain differentiates between bacterial cell wall structures based on their ability to retain or release crystal violet dye during the staining process. The process is not significantly affected by small changes in pH.
What is the gram stain reaction of strep throat?
Streptococcus pyogenes is gram positive bacteria.
How long does the smell of wood stain typically linger in a room?
The smell of wood stain can linger in a room for several days to a few weeks, depending on factors such as ventilation and the type of stain used.
