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Why protein estimation is done at 660 nanometer in folin lowry method?
Wiki User
∙ 13y ago
because folin lowry reagent has absorption maxima at 660 nm
Wiki User
∙ 13y ago
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Different methods of protein estimation?
This question has to be answered depending upon the meaning of a research project that involves proteins. The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures). Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes. On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
What is the role of heat treatment in folin-Wu method?
in folin wu's method,in second step,we add molybic acid and sodium tungstate with sodium hydroxide and water in order to form phosphomolybdic acid.we heat the whole mixture so formed vigorously to remove ammonia present in the molybic acid.if it is not removed then it can form its complex with copper sulphate (alkaline) .due to this it will become unavailable to react with the given sample of deprotinised blood.and we cant proceed the glucose estimation further.
What is the reaction mechanism of the folin ciocalteu reagent and poly phenols?
The Folin-Ciocalteu reagent which is a mixture of tungstates and molybdates works on the mechanism of oxidation-reduction reaction. The method strongly relies on the reduction of the mixture heteropolyphosphotungsates-molybdates by the phenolic compound which results in the formation of blue coloure chromogen. The phenolic compounds react with Folin-Ciocalteu reagent only under basic conditions adjusted by sodium carbonate solution. Under Basic conditions it has been observed that the phenolic compound undergoes dissociation to form a phenolate anion which reduces the Folin-Ciocalteu reagent i.e. the mixture of tungstates and molybdates rendering a blue coloured solution. The colour intensity of the formed blue chromogen can be measured by the absorbance readings using a spectrophotometer
How do you prepare neural ferric chloride solution?
The ferric chloride test is used to determine the presence or absence of phenols in a given sample. Enols give positive results as well. The bromine test is useful to confirm the result, although modern spectroscopic techniques (e.g. NMR and IR spectroscopy) are far superior in determining the identity of the unknown. The quantity of total phenols may be spectroscopically determined by the Folin-Ciocalteau assay.
What is lambda max for colorless proteins?
if the protein is colorless then it is quite tough to calculate the lambda max or absorption maxima directly (still you can check it by absorbtion maxima calculation/experiment). Otherwise, for its estimation first you have to treat it with any reagent (like folin reagent) then it will form a colored complex and then you can calculate it. For the common estimation of protein, there is a "Lowry Method" where you can calculate it on 750 wavelength.
Why folin Wu tubes are used for sugar estimation?
to prevent autooxidation of cuprous oxide by atmospheric oxygen
What are the reagents used in the estimation of antioxidants?
There are various reagents that are used in the estimation of antioxidants. Folin-Ciocalteu and DPPH are the most common reagents used in determining antioxidant activity.?æ
Different methods of protein estimation?
This question has to be answered depending upon the meaning of a research project that involves proteins. The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures). Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes. On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
How folin ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
How folin-ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
How to make in laboratory Folin and Wu's solution?
A description of the method and reagents is at this link.
What is the role of heat treatment in folin-Wu method?
in folin wu's method,in second step,we add molybic acid and sodium tungstate with sodium hydroxide and water in order to form phosphomolybdic acid.we heat the whole mixture so formed vigorously to remove ammonia present in the molybic acid.if it is not removed then it can form its complex with copper sulphate (alkaline) .due to this it will become unavailable to react with the given sample of deprotinised blood.and we cant proceed the glucose estimation further.
When did Otto Folin die?
Otto Folin died on 1934-10-23.
When was Otto Folin born?
Otto Folin was born on 1867-04-04.
When did Léopold de Folin die?
Léopold de Folin died in 1896.
When was Léopold de Folin born?
Léopold de Folin was born in 1817.
