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Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
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What do you think would be the configuration of phospholipids immersed in oil?
Oil chemists often use spectroscopy to determine total Phosphorus content. Then they can calculate content of Phospholipids based on molecular weight of expected fatty acid species. The configuration of the content immersed in oil would be the total calculated content.
What are the disadvantages of total count method by haemocytometer?
Some disadvantages of the total count method using a hemocytometer include potential variability due to human error in counting, difficulty in distinguishing between live and dead cells, and the time-consuming nature of the process. Additionally, the method may not be suitable for samples with low cell concentrations.
How much is the GC content of human genome?
The GC content of the human genome is approximately 41%. This means that guanine and cytosine nucleotides make up about 41% of the total bases in the genome.
What is the difference between moisture and loss on drying?
Moisture content refers to the total amount of water present in a material, while loss on drying is a method used to determine the amount of moisture in a sample by measuring the weight lost after drying it. Loss on drying involves heating a sample to evaporate moisture, which is different from directly measuring the total amount of water present in a material.
How many RPM and time needed to centrifuge sample in Total Carbohydrate Determination by Anthrone Method?
Typically, samples are centrifuged at around 3000 RPM for 10-15 minutes in the Total Carbohydrate Determination by Anthrone Method. However, specific protocols may vary depending on the type of centrifuge and sample being used, so it's important to follow the guidance provided by the protocol or kit instructions.
How can you calculate Gallic acid equivalents for total phenolic constituents?
The total phenolic content can be calculated as gallic acid equivalent (GAE) by the following equation: T=CxV/M. T is the total phenolic content in mg·g-1 of the extracts as GAE, C is the concentration of gallic acid established from the calibration curve in mg·ml-1, V is the volume of the extract solution in ml and M is the weight of the extract in g.
Why incubate the samples at 37C in the total phenolic assay?
Incubating the samples at 37°C in the total phenolic assay helps to promote the activity of the enzymes responsible for phenolic compound extraction and detection. This temperature mimics physiological conditions and enhances the efficiency of phenolic compound extraction from the sample matrix.
How folin ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
How do you calculate total phenolics by folin ciocalteu method?
To calculate total phenolics using the Folin-Ciocalteu method, first prepare a sample solution and a standard gallic acid solution. Mix the sample with the Folin-Ciocalteu reagent and a sodium carbonate solution, then incubate the mixture for a specified time. Measure the absorbance at 765 nm using a spectrophotometer and compare it to a standard curve of gallic acid to determine the total phenolic content, usually expressed as mg of gallic acid equivalents per gram of sample.
What is the reaction mechanism of the folin ciocalteu reagent and poly phenols?
The Folin-Ciocalteu reagent which is a mixture of tungstates and molybdates works on the mechanism of oxidation-reduction reaction. The method strongly relies on the reduction of the mixture heteropolyphosphotungsates-molybdates by the phenolic compound which results in the formation of blue coloure chromogen. The phenolic compounds react with Folin-Ciocalteu reagent only under basic conditions adjusted by sodium carbonate solution. Under Basic conditions it has been observed that the phenolic compound undergoes dissociation to form a phenolate anion which reduces the Folin-Ciocalteu reagent i.e. the mixture of tungstates and molybdates rendering a blue coloured solution. The colour intensity of the formed blue chromogen can be measured by the absorbance readings using a spectrophotometer
How do you calculate total nitrogen in organic manure?
Total nitrogen in organic manure is typically calculated by multiplying the nitrogen content percentage by the total weight of the organic manure sample. This can be determined using laboratory analysis techniques such as the Kjeldahl method or Dumas combustion method. The calculated total nitrogen content is usually expressed as a percentage of the total weight of the organic manure.
Which is the easiest method to analyze total chromium content in stainless steel wire?
a technique called x-ray flouresence is excellent. Atomic Absorption may also be used.
How can you check the solid content of Phenol Formaldehyde Resin?
The total solid content of the resin can be determined by weighing 2 grams of it in an aluminum dish. It must be placed in an oven set to 100 degrees Celsius for 2 hours, and then the dry resin can be used to calculate the solid content.
What portion of the body's total calcium content is deposited in the skeleton?
Approximately 99% of the body's total calcium content is deposited in the skeleton.
Why HCl is used in Kjeldahl method?
HCl is used in the Kjeldahl method to help digest the organic material by converting nitrogen in the sample to ammonium chloride, making it easier to measure the total nitrogen content. Additionally, HCl helps to prevent interference from non-nitrogenous compounds during the digestion process.
How correlation between brix and total sugar?
Brix measures total dissolved solids while Total sugar is only a measure of sugar content. Higher sugar content will raise the brix measurement, but higher brix measurement does not always mean there is a higher sugar content.
What is the keyword density divided by area for the given content?
To calculate the keyword density divided by area for the given content, you need to divide the number of times the keyword appears in the content by the total word count of the content. This will give you the keyword density, which is a measure of how often the keyword is used in relation to the total number of words in the content.
