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HCl is used in the Kjeldahl method to help digest the organic material by converting nitrogen in the sample to ammonium chloride, making it easier to measure the total nitrogen content. Additionally, HCl helps to prevent interference from non-nitrogenous compounds during the digestion process.
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What is the standard reference method for protein determination?
The standard reference method for protein determination is the Kjeldahl method. This method involves digesting the protein sample with concentrated sulfuric acid and then measuring the amount of nitrogen present, which is used to calculate the protein content.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
Why is HCl added for the titration of vitamin C?
HCl is added to adjust the pH of the solution, making it more acidic and promoting the conversion of ascorbic acid (vitamin C) to its oxidized form, dehydroascorbic acid, which is easily detectable by the titration method used to quantify vitamin C content.
Kjeldahl method for estimation of nitrogen in inorganic organic compound?
This is a very convenient method but its use is restricted.This method is suitable for estimating nitrogen in those organic compounds in which nitrogen is linked to carbon and hydrogen. The method is not used in the case of nitro, azo and azoxy compounds. The method is extensively used for estimated nitrogen in food, fertilizers and agricultural products.Principle:- the method is based on the fact that when the nitrogenous compound is heated with concentrated sulphuric acid in presence of copper sulphate, the nitrogen present in the compound is quantitatively converted to ammonium sulphate. The ammonium sulphate so formed is decomposed with excess of alkali and the ammonia evolved is estimated volumetrically. The percentage of nitrogen is then calculated from the amount of ammonia.
What is hydroxyzone hcl used for?
Hydroxyzine HCl is an antihistamine used to treat itching, hives, and symptoms of allergies. It can also be used for anxiety and tension as a sedative.
What is the standard reference method for protein determination?
The standard reference method for protein determination is the Kjeldahl method. This method involves digesting the protein sample with concentrated sulfuric acid and then measuring the amount of nitrogen present, which is used to calculate the protein content.
When did Johan Kjeldahl die?
Johan Kjeldahl died on 1900-07-18.
When was Johan Kjeldahl born?
Johan Kjeldahl was born on 1849-08-16.
How do you estimate proximate composition of fish?
proximate composition of fish include moisture, protein, fat, ash.moisture is estimated by oven method, protein by kjeldahl method, fat by soxhlet method ash by calcination method in the muffle furnace.
How is the Kjeldahl method used to determine the amount of protein in a food?
The Kjeldahl method determines protein content by measuring the nitrogen content in a food sample. The sample is digested with sulfuric acid, converting organic nitrogen into ammonium sulfate. After neutralization with a strong base, the ammonia released is distilled and quantified, typically using titration. The nitrogen content is then converted to protein content using a conversion factor, usually 6.25, assuming that proteins contain approximately 16% nitrogen.
Why is HCl used in sahlis method to determin hb count?
We add Hydrochloric acid (hcl) to convert hemoglobin to acid hematin which is a brownish red color, which we compare with the color of the glass of the Sahli apparatus (Hemoglobinometer). The meniscus (Glass containing the solution) should have marks thatmeasureboth the hemoglobin amount and percentage in the blood.
What is Acid hematin Method?
it is formed when hcl reacts with hemoglobin ................it is the end product
Can we Use fajans method to determined the exact concentation of a approximate 0.01 M hcl solution?
No, Fajans method is not suitable for accurately determining the concentration of a dilute 0.01 M HCl solution. Fajans method is typically used for determining the concentration of anions in a solution, specifically for halide ions in the presence of a specially selected indicator. For accurate determination of the concentration of a 0.01 M HCl solution, titration with a suitable base such as sodium hydroxide would be more appropriate.
Is Ciproflocaxin hcl used to treat BV?
Ciproflocaxin hcl isn't used to treat BV.
What is the purpose of soda lime in the detection of nitrogen in nitrogen containing compounds?
Soda lime is used to detect the presence of nitrogen in nitrogen-containing compounds through a process called the Kjeldahl method. It is used to absorb the evolved ammonia gas during the digestion of the sample, which is then released by heating the soda lime and can be quantitatively determined. This method helps in determining the nitrogen content in organic compounds.
Why is HCl added for the titration of vitamin C?
HCl is added to adjust the pH of the solution, making it more acidic and promoting the conversion of ascorbic acid (vitamin C) to its oxidized form, dehydroascorbic acid, which is easily detectable by the titration method used to quantify vitamin C content.
Kjeldahl method for estimation of nitrogen in inorganic organic compound?
This is a very convenient method but its use is restricted.This method is suitable for estimating nitrogen in those organic compounds in which nitrogen is linked to carbon and hydrogen. The method is not used in the case of nitro, azo and azoxy compounds. The method is extensively used for estimated nitrogen in food, fertilizers and agricultural products.Principle:- the method is based on the fact that when the nitrogenous compound is heated with concentrated sulphuric acid in presence of copper sulphate, the nitrogen present in the compound is quantitatively converted to ammonium sulphate. The ammonium sulphate so formed is decomposed with excess of alkali and the ammonia evolved is estimated volumetrically. The percentage of nitrogen is then calculated from the amount of ammonia.
