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The Kjeldahl method determines protein content by measuring the nitrogen content in a food sample. The sample is digested with sulfuric acid, converting organic nitrogen into ammonium sulfate. After neutralization with a strong base, the ammonia released is distilled and quantified, typically using titration. The nitrogen content is then converted to protein content using a conversion factor, usually 6.25, assuming that proteins contain approximately 16% nitrogen.

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2mo ago

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What is the standard reference method for protein determination?

The standard reference method for protein determination is the Kjeldahl method. This method involves digesting the protein sample with concentrated sulfuric acid and then measuring the amount of nitrogen present, which is used to calculate the protein content.


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How can a scale accurately measure protein content in a sample?

A scale can accurately measure protein content in a sample by using a method called the Kjeldahl method. This method involves weighing the sample, digesting it with acid to release nitrogen from proteins, and then measuring the nitrogen content to calculate the protein content. The scale ensures precise measurement of the sample weight, which is crucial for accurate protein content determination.


How do you test for protein in food?

Protein in food can be tested using methods such as the Kjeldahl method, Dumas method, or the Bradford assay. These methods involve measuring nitrogen content, which is then converted to protein content using a conversion factor. Commercially-available test kits and equipment are also available for protein analysis in food.


What is protein estimation?

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Why does milk test positive for protein?

Milk tests positive for protein because it naturally contains two main types of protein: casein and whey. These proteins can be detected using specific testing methods like the Kjeldahl method or the Bradford assay. The presence of these proteins makes milk a good source of dietary protein.


Why HCl is used in Kjeldahl method?

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How iodimetry work?

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