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Incubating the samples at 37°C in the total phenolic assay helps to promote the activity of the enzymes responsible for phenolic compound extraction and detection. This temperature mimics physiological conditions and enhances the efficiency of phenolic compound extraction from the sample matrix.
What else can I help you with?
How can you calculate Gallic acid equivalents for total phenolic constituents?
The total phenolic content can be calculated as gallic acid equivalent (GAE) by the following equation: T=CxV/M. T is the total phenolic content in mg·g-1 of the extracts as GAE, C is the concentration of gallic acid established from the calibration curve in mg·ml-1, V is the volume of the extract solution in ml and M is the weight of the extract in g.
What is the reaction mechanism of the folin ciocalteu reagent and poly phenols?
The Folin-Ciocalteu reagent which is a mixture of tungstates and molybdates works on the mechanism of oxidation-reduction reaction. The method strongly relies on the reduction of the mixture heteropolyphosphotungsates-molybdates by the phenolic compound which results in the formation of blue coloure chromogen. The phenolic compounds react with Folin-Ciocalteu reagent only under basic conditions adjusted by sodium carbonate solution. Under Basic conditions it has been observed that the phenolic compound undergoes dissociation to form a phenolate anion which reduces the Folin-Ciocalteu reagent i.e. the mixture of tungstates and molybdates rendering a blue coloured solution. The colour intensity of the formed blue chromogen can be measured by the absorbance readings using a spectrophotometer
Which two samples of gas at STP contain the same total number of molecules?
Two samples of gas at STP containing the same total number of molecules would have equal volumes, as Avogadro's Principle states that equal volumes of gases contain equal numbers of molecules at the same temperature and pressure. Thus, 1 mole of any gas at STP will have the same number of molecules as 1 mole of any other gas at STP.
How do they know how much coal is in a coal mine?
They core drill from the surface down and remove samples. These core samples give them the coal seam thickness and coal quality. Multiply the seam thickness by total area and you have the coal reserves, typically measured in acre-feet.
How do you calculate hplc assay and hplc purity?
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
What are the reagents used in the estimation of antioxidants?
Common reagents used in the estimation of antioxidants include DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, Folin-Ciocalteu reagent, and Trolox standard. These reagents are utilized in assays such as DPPH radical scavenging assay, total phenolic content assay, and oxygen radical absorbance capacity (ORAC) assay to measure the antioxidant capacity of compounds.
How can you calculate Gallic acid equivalents for total phenolic constituents?
The total phenolic content can be calculated as gallic acid equivalent (GAE) by the following equation: T=CxV/M. T is the total phenolic content in mg·g-1 of the extracts as GAE, C is the concentration of gallic acid established from the calibration curve in mg·ml-1, V is the volume of the extract solution in ml and M is the weight of the extract in g.
How do you calculate total phenolics by folin ciocalteu method?
To calculate total phenolics using the Folin-Ciocalteu method, first prepare a sample solution and a standard gallic acid solution. Mix the sample with the Folin-Ciocalteu reagent and a sodium carbonate solution, then incubate the mixture for a specified time. Measure the absorbance at 765 nm using a spectrophotometer and compare it to a standard curve of gallic acid to determine the total phenolic content, usually expressed as mg of gallic acid equivalents per gram of sample.
What does Assay of psa total 84153-GA mean on a lab bill?
"Assay of PSA total 84153-GA" means the patient had a PSA (prostate specific antigen) test done. This is a common screening test in men in middle age and older. 84153 is the CPT code for the test.
How folin-ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
Test samples at 0.17 gram 0.204 gram and 2.3 grams What is the total weight of the three samples?
2.674 g
What is the formula for calculating assay and potency?
Assay is typically calculated as the percentage of the active ingredient present in a sample compared to the total sample weight. Potency is a measure of the strength or concentration of the active ingredient in a sample relative to a standard reference. The formula for assay and potency calculations may vary depending on the specific assay method and the properties of the substance being analyzed.
A chemist weighed three separate test samples at 0.17 gram 0.204 gram and 2.3 grams What is the total weight of the three samples?
2.674 grams
Can a robin have 7 eggs?
yes, even more during a certain period. Total eggs that incubate are usually much great than 7.
Why is the total protein are measured at 595 nm in the Bio-Rad assay?
The Bio-Rad protein assay measures the total protein content in a sample at 595 nm because this wavelength corresponds to the absorption peak of protein-bound Coomassie Brilliant Blue dye. When proteins are present in the sample, they bind to the dye, causing a shift in absorbance at 595 nm, which is used to accurately quantify the protein concentration.
What is icteric index and how can measure icetric index?
The icteric index is a clinical indicator used to assess the degree of jaundice, specifically by measuring bilirubin levels in the blood. It is calculated by taking the ratio of total bilirubin to the direct (conjugated) bilirubin levels, usually expressed as a percentage. To measure the icteric index, blood samples are taken and analyzed using a laboratory assay that quantifies the concentrations of total and direct bilirubin, allowing healthcare providers to evaluate liver function and diagnose potential liver diseases.
