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Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
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What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
The cell's DNA does not contain the restriction site.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
What do biologists use to cut DNA into smaller fragments?
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences called restriction sites.
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
What do you use to cut DNA strands for DNA fingerprints?
A Retsriction enzyme endonuclease is an enzyme that is used to cut DNA strands (both single and double strands) during finger printing at the DNA recognition sites known as restriction sites.
Where do they cut the DNA strand?
They direct a specific Restriction Enzyme to cut the Dna Exactly where required.
What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
The cell's DNA does not contain the restriction site.
What type of enzyme is used to fragment dna?
restriction enzymes are used to cut DNA.
Which enzyme do scientist use to cut genes out of strands of DNA?
restriction endonuclease
Which enzyme do scientist use to cut out strands of DNA?
They are called restriction enzymes and there are all sorts depending on the sequence of DNA they are trying to cut
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
Can Restriction Enzymes be used on all DNA?
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
