0
The first chromatography used was with polar stationary phase and non polar mobile phase, called normal phase. So, later when this was reversed by using polar mobile phase and non polar stationary phase was called reversed phase.
Although reversed phase implies that it is less used, it is not the case. RPLC rose to success around the 1970s as NPLC dropped off.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
In what phase the reverse of prophase?
The first chromatography used was with polar stationary phase and non polar mobile phase, called normal phase. So, later when this was reversed by using polar mobile phase and non polar stationary phase was called reversed phase. Although reversed phase implies that it is less used, it is not the case. RPLC rose to success around the 1970s as NPLC dropped off.
Why is there an Iron chloride impurity in chromatography experiment?
The water used to dilute HCL may contain Iron chloride as an impurity so the solution you prepare as a mobile phase is contaminated with iron chloride.
What is the type of switch that reverses the rotation of a single phase motor?
If you are talking about a switch that is used to change the direction that a single phase motor turns from clockwise to counter clockwise, the switch is called a forward - reverse drum switch. By reversing any two of the three phase legs that feed the 3 phase motor. <<>> On a single phase motor a drum switch is used. This switch reverses the start winding so that when voltage is applied to the motor it will revolve in the reverse direction.
Why do you find paper chromatography so interesting?
It's not interesting.
What is the root meaning of chromatography?
Chromatography comes from the Greek chrom- meaning "colored" and graph- meaning "writing" so it literally means "colored writing".
In what phase the reverse of prophase?
The first chromatography used was with polar stationary phase and non polar mobile phase, called normal phase. So, later when this was reversed by using polar mobile phase and non polar stationary phase was called reversed phase. Although reversed phase implies that it is less used, it is not the case. RPLC rose to success around the 1970s as NPLC dropped off.
What is the basic physical principle that chromatography is based upon?
The principle behind Chromatography is :The rate of migration of the solute depends upon the rate of interaction of the solute with the two phases, one being the mobile phase and the other stationary phase as the compounds travel through the supporting medium.
Why is there an Iron chloride impurity in chromatography experiment?
The water used to dilute HCL may contain Iron chloride as an impurity so the solution you prepare as a mobile phase is contaminated with iron chloride.
How does chiral Hplc work?
As a very crude analogy, think of a playground slide with dents in it that are the size and shape of a left shoe print. If we put some shoes at the top and let them slide down, the left shoes will be more likely to get stuck in the dents than the right shoes (which don't fit as well and therefore slide over them more easily).
What is called when you think the opposite of what you want so you get it?
Reverse Psychology
What is the type of switch that reverses the rotation of a single phase motor?
If you are talking about a switch that is used to change the direction that a single phase motor turns from clockwise to counter clockwise, the switch is called a forward - reverse drum switch. By reversing any two of the three phase legs that feed the 3 phase motor. <<>> On a single phase motor a drum switch is used. This switch reverses the start winding so that when voltage is applied to the motor it will revolve in the reverse direction.
How can paper chromatography be used to separate pigments based on their chemical and physical properties?
It is an appropriate technique to use because it separates the pigments, so one can see which pigments are present, even if some pigments are normally hidden to the naked eye.
When human DNA is analysed using chromatography what are the results called?
I so want to say "genetic fingerprint" but I am crushed by the poor/overly brief wording of the question that perhaps I won't. Do we know what type of chromatography is being used? Do we know any more details of the experiment?
Why do you find paper chromatography so interesting?
It's not interesting.
Use of gas chromatography in pharmaceutical industry?
Chromatography is a laboratory process that occurs in several steps and is used to separate mixtures of various chemicals into their individual components. The governing principle of chromatography is that different chemicals in a mixture have different degrees of dissolving in a liquid or sticking to a solid surface. In other words, chromatography can identify a chemical and separate it from a dense mixture of other chemicals and show it on a surface. Various chemicals in a mixture have different sticking ability on a surface. By varying this process in many ways, the chromatography technique can be used to separate any amount of quantities ranging from micrograms (in laboratories) to tons (in chemical plants). There are various chromatography procedures that have become popular since the invention of chromatography by Russian botanist, Mikhail Semyonovich Tsvet, in 1901. In the chromatography processes, a stream of liquid, that is called mobile phase, is made to flow through a tube known as column, and it is packed with porous solid material, called the stationary phase. The sample of the mixture that is to be analyzed is sent through the mobile phase and as the mixture proceeds in the tube, the compounds are separated. Chromatography is preferred over many other techniques as it doesn't cause any molecular changes in the composition of the chemicals involved. Uses of Chromatography Chromatography has evolved to be one of the most widely used chemical techniques to separate particles and contaminates in chemical plants. For example, in the chemical industries, pesticides and insecticides like DDT in the groundwater and PCBs (Polychlorinated biphenyls) are removed by the process of chromatography. As a major testing tool, chromatography is used by government agencies to separate toxic materials from the drinking water and also to monitor air quality. Chromatography is used by pharmaceutical companies to prepare large amounts of pure materials that are further required in making medicines. Also, it is used to check the presence of any contamination in the manufactured compounds. In the field of organic chemistry and pharmacy, chiral compounds are very close to each other in terms of atomic or molecular weight, element composition, and the physical properties. However, they exist in two different forms, called the enantiomers and optical isomers. Both these compounds though may appear to be same, have very different chemical properties. So, in pharmacy, chromatography becomes crucial to analyze the exact chiral compound so that correct medicines can be manufactured. For instance, a compound called thalidomide has two optical isomers and one of the isomers can cause birth defect if a pregnant women consumes it in early stages of pregnancy. So, it is important to carefully separate the isomers. Chromatography is used as a technique to separate the additives, vitamins, preservatives, proteins and amino acids. Some other uses are in the detection of drugs or medications in the urine and the separation of traces of chemicals in the case of fire in houses or buildings. It is also very popular in forensic science for investigative purposes. Chromatography technology has gained immense industrial popularity in the past few decades as it can separate chemicals that just differ even in their atomic orientations in space.
What is the root meaning of chromatography?
Chromatography comes from the Greek chrom- meaning "colored" and graph- meaning "writing" so it literally means "colored writing".
Uses for paper chromatography?
They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.
