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Why should you not blow out the small amount of solution that remains in a pipette when measuring liquid?

The calibration of a pipette is made taking into account this small volume.


When can a mouth used to suck liquid into the pipette?

The mouth should never be used to suck liquid into a pipette. This is because it can be dangerous due to potential exposure to harmful chemicals, contamination of the sample, and inaccurate measurements. It is recommended to use a bulb or pipette aid to safely draw liquid into the pipette.


Should you expect to see a meniscus when using a pipette?

Yes, you should expect to see a meniscus when using a pipette, as it is a natural phenomenon that occurs due to surface tension. The meniscus is the curved surface of a liquid in the pipette, and when measuring liquids, it's important to read the volume at the bottom of the meniscus for accuracy. This ensures precise measurements, especially in scientific experiments.


Why should we let the pipette dispense on its own?

Forcing liquid out of a pipette - could mean it splashes the area. This is dangerous - especially if the liquid happens to be corrosive or toxic.


Why should you not blow out the small amount of solution that remains in pipette when measuring liquids?

The calibration of a pipette is made taking into account this small volume.


Which measuring instrument should the biologist use to add the enzyme solution to the test tubes?

A pipette or a micropipette.


Why is it important to calibrate a pipette prior to it being used to make up standard solution?

Pipettes are used to move specific volumes of fluid between containers. For very small volumes, or very precise applications, a large error in the desired fluid volume can have a very large impact. Calibrating a pipette ensures that the volume desired is the volume collected. Proper sealing of pipette tips can also have a large impact on fluid volumes collected.


You are using a pipette that has a mark every 0.1 mL. If you want to measure 5.15 mL of liquid using this pipette to what level should you fill the pipette?

The bottom of the meniscus should be halfway between the 5.1-mL mark and the 5.2-mL mark


If you are trying to pipette an unknown liquid with a pipetman and the liquid keeps running out of the tip before you can transfer it what are two possible reasons for this what can you do to remedy t?

The unknown liquid could have a low suface tension like may organic liquids. Also, the plunger of the pipet could be being pushed all the way down when extracting the liquid ( should be at first stop) so it leaks out before the transfer is complete. To avoid this, pipet some liquid in and blow it out by pushing the plunger all the way down (second stop). Then draw up some more of the liquid and it should not leak.


Liquid volume should be measured in what?

with measuring tools


How do you measure the volume of liquid in a pipette?

To measure the volume of liquid in a pipette, you should read the volume markings on the side of the pipette. Ensure the liquid level is at the calibration mark for an accurate measurement. It is essential to keep the pipette vertical during measurement to prevent errors in volume readings.


What type of measuring device should be used to measure ml of liquid for compounding?

A graduated cylinder or a precision volumetric flask is ideal for measuring milliliters of liquid when compounding. Graduated cylinders provide a clear scale for accurate measurement, while volumetric flasks ensure precise volume for solutions. For smaller quantities, a syringe or a pipette can also be used for more accurate dosing. Always ensure that the measuring device is calibrated and appropriate for the volume being measured.