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If you are trying to pipette an unknown liquid with a pipetman and the liquid keeps running out of the tip before you can transfer it what are two possible reasons for this what can you do to remedy t?
Inesarch
The unknown liquid could have a low suface tension like may organic liquids. Also, the plunger of the pipet could be being pushed all the way down when extracting the liquid ( should be at first stop) so it leaks out before the transfer is complete. To avoid this, pipet some liquid in and blow it out by pushing the plunger all the way down (second stop). Then draw up some more of the liquid and it should not leak.
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