A nutrient medium is used.
a sterile one
Fermentation processes can be carried out in two ways; surface or submerged production. Submerged production began in the 1930's and is the main method used today. The sterile medium and mould are added to a large tank where it is constantly mixed and a supply of sterile air is bubbled through (since the fermentation process is aerobic). The strain of mould used is different to that in surface production. The process can be used to make products such as citric acid, glycerol or lactic acid.
Divide your agar plate into 4 quadrants, A sterile inoculating loop is dipped ito mixed culture, streaked in a pattern over surface of nutrient medium. As pattern transferred, bacteria are rubbed off into the medium. sterilize in between quadrants, the last cells to be rubbed off the loop are far enough apart to grow into isolated colonies. Pick up a colony with sterile loop and transfer into test tube of nutrient medium to form a pure culture containing only one type of bacterium.
because it is important that in nutrient media to grow up only bacteria that may be present in your mouth.In tha case tha swabs are not sterile in nutrient media will growth mouth and swab bacteria and the will give a flas positive result.
The medium is a sterile substrate that takes the place of soil; it provides a place for the plants to grow in as well as a medium through which the nutrient solution can pass. Hydroponics employs a sterile medium, one that does not interact with the plants - perlite, vermiculite, inert stone or rock wool. Hydroponic gardening system that is based on water, not soil. So container is a hydroponic growing medium. There are various types of hydroponic growing mediums like Oasis Cube, Coconut Fiber, Fillers, Ebb and Flow System and aeroponics.
A nutrient medium is used.
simply agar medium
a sterile one
Fermentation processes can be carried out in two ways; surface or submerged production. Submerged production began in the 1930's and is the main method used today. The sterile medium and mould are added to a large tank where it is constantly mixed and a supply of sterile air is bubbled through (since the fermentation process is aerobic). The strain of mould used is different to that in surface production. The process can be used to make products such as citric acid, glycerol or lactic acid.
simply agar medium
Divide your agar plate into 4 quadrants, A sterile inoculating loop is dipped ito mixed culture, streaked in a pattern over surface of nutrient medium. As pattern transferred, bacteria are rubbed off into the medium. sterilize in between quadrants, the last cells to be rubbed off the loop are far enough apart to grow into isolated colonies. Pick up a colony with sterile loop and transfer into test tube of nutrient medium to form a pure culture containing only one type of bacterium.
Hydroponics substitute a nutrient medium in place of soil. for the plants to root and grow. The nutrient medium may be either a water solution or an inert material.
sterile
because it is important that in nutrient media to grow up only bacteria that may be present in your mouth.In tha case tha swabs are not sterile in nutrient media will growth mouth and swab bacteria and the will give a flas positive result.
Usually a sterile mix of vermiculite, pearlite, moss, and steralized soil.
Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.