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the color of the salt prevents the recognition of the color produced in the reaction. The presence of ammonium salts interferes with the test

hope usefull :D

source: Experimental Organic Chemistry - James F. Norris, Ph.D. in chapter 26

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Q: Why should excess cuso4 not be used in biuret test?
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Would albumin give a positive biuret test?

Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.


How many of these chemical groupings must be present in a molecule to give a positive biuret test?

you should have at least 2 of these,to obtain a positive result in Biuret's test. because biuret test is a test used to detect if there is peptide linkages.


What substance is used as a control in a Biuret test?

Biuret reagent is made up of sodium hydroxide and copper sulphate.


What color will a peptide bond be after using the Biuret test?

in biuret test the presence of peptide bonds gives a violet color. but it should be noted that a minimum of two peptide bonds should be present in the protein to get a positive answer to this test.


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Detect peptide bond?

by conducting the biuret test. the positive result of biuret test will give you the purple blue ring on top of the solution.(albumen/milk + cuso4 +NaOH)


Is biuret test suitable to test the presence of proteins in the urine?

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Does pepsin test positive in biuret test?

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What test would you run to test for the presence of a polypeptide?

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What might Biuret reagent test in a practical real life situation?

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