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Q: Why should we carefully lower the coverslip onto the specimen during a wet mount preparation?
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What is OIO in microscope?

oIO, stands for oil immersion objective. it was discovered during 1870. the OIO, magnifies the actual size of the specimen , 100 times, with the aid of placing a drop of ceddar oil at the top of the coverslip in slide. Remember that the OIO objective must touch the surface of the oil.


Which of the objectives comes closest to a specimen and is most likely to break a slide if proper precautions are not take?

The objective lens on a microscope that comes closest to the specimen and is most likely to break a slide if proper precautions are not taken is the high-power objective. High-power objectives are designed for high magnification, typically in the range of 40x to 100x, and they have a very short working distance. The working distance is the distance between the objective lens and the specimen. Because the high-power objective has such a short working distance, it means that it needs to be very close to the specimen being observed. If the slide or coverslip is too thick or if there's an uneven or raised area on the slide, there's a risk that the objective lens may come into contact with the slide, potentially causing damage to both the lens and the slide. This is why it's crucial to handle slides carefully and ensure they are prepared with the appropriate thickness and flatness to avoid damaging the high-power objective and obtaining clear images during microscopy.


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