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You should use the lowest value lens. It is the shortest one.
It should be stored with the low-power objective in place.
The low power or lowest power objective
No
A microscope should be stored in low power objective lens because the lens can scratch against the lens.
Ocular Lens (eyepiece) or Stage
When focusing the microscope downward, you take the chance of damaging the expensive objective lens by crushing the slide trying to get it.
When starting a microscopic study,always examine under LPO first.Move the slide and get a good general view of the specimen.
Scanner or 4x objective. This is to not confuse our eyes on the magnification that we will see.
A Magnifying Glass
According to my neurons that the distance from slide to objective lens should be increase rather than decrease when coarse focusing with the scanner and LPO to obtain a clearer and more focus structure of a certain specimen.. U must ensure that the coarse adjustment knob is at it's extent and then turn on the fine adjustment knob clockwise for the easier visibility of the specimen =)) - VALERIC ACID ...sPCUTE ..=)
When a very close view of the specimen is necessary. You can focus in on one part of the specimen.
You should use the lowest value lens. It is the shortest one.
If you move closer while looking thru the lens, there is a danger of cracking the slide, especially using coarse adjustment.
What do you do when you want to go into a new room and sit down? Do you crawl around the floor until you bump into a chair? No. You stand up an look around the room. You scan the room. When you get close to the chair, you look closer to make sure no one else is sitting in it or to see nothing is resting on the cushion. You use distance vision to find the chair. Then you examine it closely when you are close. Finally you check it to make it will support your weight. Then you sit down. Then you sit down. You start with the lens with the lowest magnification to locate the specimen. You move the specimen into the center of view. etc.
It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing. It also reduces the chance of crushing the specimen and/or breaking the slide or the lens as the LPO is further away from the slide than the HPO.
Try it any other way and you'll see. The scanning objective has the largest field of view, the greatest depth of field, and the greatest working distance. It is the most forgiving objective. It is the easiest to find the specimen and focus on it.