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Why some spots overlap in chromatography?

Updated: 12/5/2022
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Sharonwoo

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Q: Why some spots overlap in chromatography?
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What happens when paper chromatography is left in the solution?

all of the spots migrate to the top, and you will not be able to tell them apart anymore.


What would be the result if the spots were made to large in a thin layer chromatography experiment?

it would be difficult to read as they would merge


Why is it important to keep the spots applied to TLC slides for chromatography as small as possible?

The components may not separate properly, because the solvent will overload


What would happen if you submerge the chromatography paper above the pencil line?

Try and see: if it wouldn't be above the level of solvent, the spots will run off.


Why do some region overlap?

they dont


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What are some synonyms of the term 'overlap'?

Some synonyms of the term overlap are extend along and fold over. The word overlap is defined as to lie over something else or the winged or extended part of an object.


What are 3 types of chromatography?

1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography


What are some possible alternatives for paper chromatography?

Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works.


Calibration of gas chromatography?

What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography? What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography?


What is grass chromatography?

There is no such thing as grass chromatography. there is only High Performance Liquid Chromatography, Gas Chomatography, and Paper Chromatography.


Why should the amino acids spots not be submerged within the solvent?

I assume you are talking about thin-layer chromatography. If the spots are submerged in the solvent, they will dissolve into it and become so diluted that they will most likely be undetectable. Plus, they will elute as a band, not as a spot. Your solvent will also be contaminated.