Because electron microcopy images are not taken 'in situ'. In other words, to capture an image using an electron microcope, the cells are often heavily treated in order to allow the image to be be generated. These treatments include freezing, fracturing and/or coating with heavy metals. Such treatments kill the cells, and therefore we aren't capturing an image of a living cell, but a cell we have killed and treated. Because these treatments are not encountered in the 'normal day-to-day life' of the living cells, we can't say for sure that they don't affect the structure of the cell we're trying to take the picture of.
Field Emission Scanning Electron Microscopy
Transmission electron microscopy
Transmission electron microscopy
Lewis dot structures.
Biochemically and using electron microscopy
Field Emission Scanning Electron Microscopy
Robert F. Bils has written: 'Electron microscopy' -- subject(s): Electron microscopy, Laboratory manuals, Microscopy, Electron
Jan Vincents Johannessen has written: 'Electron Microscopy in Human Medicine: Part A' 'Diagnostic electron microscopy' -- subject(s): Case studies, Electron microscopic Diagnosis, Electron microscopy
No
Transmission electron microscopy
Electron microscopy; Scanning Electron Microscopes (SEM) and Transmission Electron Microscopes (TEM). The vacuum required for electron microscopy to work correctly precludes the observation of living organisms. Biological samples must be dried then coated with a conductive metal.
Alex Ferenczy has written: 'Female reproductive system: dynamics of scan and transmission electron microscopy' -- subject(s): Atlases, Cytology, Diagnosis, Diseases, Electron microscopic Diagnosis, Female Generative organs, Female Genitalia, Microscopy, Electron, Scanning, Scanning electron microscopy, Transmission electron microscopy
Electron microscopy gives higher resolution, but it's expensive, slow, and cumbersome. And for many things, it's not needed.
Transmission electron microscopy
transmission electron microscopy
Transmission electron microscopy
Manfred von Heimendahl has written: 'Electron microscopy of materials, an introduction' -- subject(s): Electron microscopy