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Because electron microcopy images are not taken 'in situ'. In other words, to capture an image using an electron microcope, the cells are often heavily treated in order to allow the image to be be generated. These treatments include freezing, fracturing and/or coating with heavy metals. Such treatments kill the cells, and therefore we aren't capturing an image of a living cell, but a cell we have killed and treated. Because these treatments are not encountered in the 'normal day-to-day life' of the living cells, we can't say for sure that they don't affect the structure of the cell we're trying to take the picture of.

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Q: Why the images revealed by electron microscopy might not accurately represent structures in living cells?
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