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Orange Juice is bitter after a few hours because limonin was formed. Usually limonin is not present in the orange, but forms in a few hours after the juice has been squeezed. The process can be accelerated by heat ( oh! Malaysia's weather!)

Limonin was discovered in 1841 in some orange seeds by S. Bernay. Then, Ralph Higby isoalted limonin from the orange juice in 1938. However, as there is no limonin found in a fresh orange, the mystery was only solved in 1969 when Vincent Maier identified LARL( a tasteless substance) as the precursor that give rise to limonin.

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Q: Why the orange juice become bitter if left in room temperature for some time?
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