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How does one apply the HPLC princple?
The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Where is HPLC chromatography used in industry?
HPLC chromatography is widely used in industries such as pharmaceuticals, food and beverage, and environmental analysis for separating, identifying, and quantifying compounds in complex mixtures. It is commonly used for quality control testing, analyzing drug potency, detecting impurities, and monitoring environmental contaminants.
What is the procedure for hplc instrument calibration for petrochemical industry?
The calibration procedure for HPLC (High-Performance Liquid Chromatography) in the petrochemical industry involves preparing standard solutions of known concentrations of target compounds. These standards are injected into the HPLC system, and the resulting chromatograms are used to generate calibration curves by plotting peak area or height against concentration. The instrument is then adjusted to ensure that the response is linear over the desired concentration range. Regular maintenance and checks are also performed to ensure consistent performance and accuracy throughout the calibration process.
Is hplc chromatography reliable?
Yes, HPLC chromatography is a highly reliable technique for separating and analyzing compounds based on their physical and chemical properties. It is widely used in various industries such as pharmaceuticals, food testing, environmental analysis, and more due to its high sensitivity, accuracy, and reproducibility.
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is the importance of establishing a reliable HPLC calibration curve in analytical chemistry?
Establishing a reliable HPLC calibration curve in analytical chemistry is important because it allows for accurate quantification of compounds in a sample. The calibration curve helps to determine the relationship between the concentration of a compound and its response in the HPLC system, ensuring precise and reliable measurements. This is crucial for ensuring the validity and accuracy of analytical results in various fields such as pharmaceuticals, environmental monitoring, and food testing.
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
