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Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Which type of samples analysed by hplc?
The samples that are not volatile and are soluble in the mobile phase.
Why water is not used in normal phase hplc analysis?
In normal-phase chromatography, the stationary phase is polar and the mobile phase is a mixture of non-polar solvents such as hexane and slightly more polar solvents such as isopropanol. water is the most polar solvent of all solvents. If you use water as a mobile phase, the polar analytes will remain dissolved in water and there will be no retention of analytes on the stationary phase. If there is no retention on stationary phase, there is no separation
What is the H.P.L.C. chromatography?
HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
How do you estimate Serratiopeptidase in Pharmaceutical products Is there a HPLC method available?
MOBILE PHASE A(METHANOL:ACETONITRILE), 60:40V/V ::MOBILE PHASE B (PHOSPHATE BUFFER pH 7)= 50:50V/V
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Which type of samples analysed by hplc?
The samples that are not volatile and are soluble in the mobile phase.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Why water is not used in normal phase hplc analysis?
In normal-phase chromatography, the stationary phase is polar and the mobile phase is a mixture of non-polar solvents such as hexane and slightly more polar solvents such as isopropanol. water is the most polar solvent of all solvents. If you use water as a mobile phase, the polar analytes will remain dissolved in water and there will be no retention of analytes on the stationary phase. If there is no retention on stationary phase, there is no separation
What is the H.P.L.C. chromatography?
HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
How HPLC is used as separation technique?
it used a mobile phase to carry a sample through a tube containing a stationary phase which is a liquid coated on solid particles.
How do you estimate Serratiopeptidase in Pharmaceutical products Is there a HPLC method available?
MOBILE PHASE A(METHANOL:ACETONITRILE), 60:40V/V ::MOBILE PHASE B (PHOSPHATE BUFFER pH 7)= 50:50V/V
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
What is difference between isocratic and gradient hplc?
in isocretic system we use only single mobile phase but in gredent system we use two or more mobile phases.
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
What is the systm volume and what is the dead volume and what is the wide volume and what is the delay volme in HPLC?
system volume is amount of mobile phase travel from reservior to tip of the colomn . dead volume is the amount of mobile phase present in colomn. delay volume is amount of mobile phase used in at the time of injection and the time of detection of chromatogram.
