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The lower the phenol coefficient is the stronger the disinfectant is?
No,, the higher the phenol coefficient the stronger the disinfectant
Evaluate the effectiveness of a disinfectant with a phenol coefficient of 40?
A disinfectant with a phenol coefficient of 40 indicates that the chemical agent under study is 40 times as effective as phenol.
Why FeCl2 is not used to identify phenol?
Because it's a test for phenols or enols
What eles can you use for phenol red?
cabbage juice...
What is the best solvent for phenol for use in Gas Chromatography?
water
The lower the phenol coefficient is the stronger the disinfectant is?
No,, the higher the phenol coefficient the stronger the disinfectant
Evaluate the effectiveness of a disinfectant with a phenol coefficient of 40?
A disinfectant with a phenol coefficient of 40 indicates that the chemical agent under study is 40 times as effective as phenol.
What is the molar extinction coefficient of Phenol red at 610NM?
Molar extinction coefficient of phenol ret at 610nM is 22 mM-1 cm-1
Phenol coefficient test in microbiology?
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
What is the use of chick-Martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
What is the use of chick martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Suggest a chemical test(s) to distinguish ethanol and phenol. Include the reagents reaction conditions and observations. And the equation?
Phenol solution, or phenol directly from the manufacturer?If the phenol is in the form it comes in from the factory, no chemical test is necessary. Phenol is a white crystalline solid.If it's dissolved in water, the easiest test is pH. Phenol solution is more acidic than ethanol. So...whatever you use for a pH test will do ya.
When do you use methyl red instead of phenol red?
You use methyl red as a ph indicator for for the MRVP test.
But not after 5min using phenol coefficient why?
because one bacterium takes 20 minuets to replicate.
How do you pass alcohol evaluation test?
Defects of the alcohol - coefficient tests : 1- The choice of organism . 2- The choise of phenol as standard. 3- The subculturing process . 4- The subculture medium . 5- The reaction medium . 6- The use of one tempreture. 7- The use of one concentration .
Does phenol give a positive biuret test?
No. The Biuret test only gives a positive result if a peptide bond exists. Phenol doesn't have a peptide bond, so it will not give a positive test.
HOW IS IT POSSIBLE FOR AN ORGANISM TO BE POSITIVE FOR GLUCOSE USE IN A PHENOL RED TEST BUT NEGATIVE IN THE METHYL RED TEST?
Methyl red differs from Phenolred (which is used in the fermentation test and the MSA plates) in that it is yellow at pH 6.2 and above and red at pH 4.4 and below. Phenol red turns yellow below a pH of 6.8. If you get a positive with methyl red and a negative with phenol red, the pH has to be between 6.7 and 6.1.
