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Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
What else can I help you with?
What is the intra-assay coefficient of variation for this particular experiment?
The intra-assay coefficient of variation for this experiment is a measure of the variability within the same assay or test, indicating how consistent the results are.
HOW IS IT POSSIBLE FOR AN ORGANISM TO BE POSITIVE FOR GLUCOSE USE IN A PHENOL RED TEST BUT NEGATIVE IN THE METHYL RED TEST?
Methyl red differs from Phenolred (which is used in the fermentation test and the MSA plates) in that it is yellow at pH 6.2 and above and red at pH 4.4 and below. Phenol red turns yellow below a pH of 6.8. If you get a positive with methyl red and a negative with phenol red, the pH has to be between 6.7 and 6.1.
What is carbolfuchsin?
This compound is basic.
What is the branches of the microbiology?
They study the biology of tiny organisms, such as protists bacteria and other single celled organism's Micro = small, biologist = someone who studies biology. 'Scientists who study the structure and the function of cells'
What is urease test in microbiology?
When determining the ability of a bacteria to degrade urea by means of the enzyme urease. To diagnose H. pylori and identification of the genus Proteus, Providentia, Kelbsiella, and Morganella by their fast urease activity.
What is the phenol coefficient?
The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
The lower the phenol coefficient is the stronger the disinfectant is?
No,, the higher the phenol coefficient the stronger the disinfectant
Evaluate the effectiveness of a disinfectant with a phenol coefficient of 40?
A disinfectant with a phenol coefficient of 40 is highly effective compared to phenol as a standard. It means that it is 40 times more effective than phenol at killing microbes. This high coefficient indicates strong germicidal properties and efficiency in disinfecting surfaces.
What is the molar extinction coefficient of Phenol red at 610NM?
Molar extinction coefficient of phenol ret at 610nM is 22 mM-1 cm-1
What is the use of chick-Martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
What is the use of chick martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Does phenol give a positive biuret test?
No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.
What increase absorbance of phenol in ethanol?
Possible factors that can increase the absorbance of phenol in ethanol are: increasing the concentration of phenol in the solution, using a higher path length cuvette for measurement, and selecting a wavelength for measurement where phenol has a higher molar absorptivity coefficient.
Suggest a chemical test(s) to distinguish ethanol and phenol. Include the reagents reaction conditions and observations. And the equation?
Phenol solution, or phenol directly from the manufacturer?If the phenol is in the form it comes in from the factory, no chemical test is necessary. Phenol is a white crystalline solid.If it's dissolved in water, the easiest test is pH. Phenol solution is more acidic than ethanol. So...whatever you use for a pH test will do ya.
But not after 5min using phenol coefficient why?
because one bacterium takes 20 minuets to replicate.
In mirobiology what is a test tube rack?
microbiology classification microbiology classification
What is the name of the pH indicator used in microbiology?
The pH indicator commonly used in microbiology is phenol red. It changes color depending on the pH of the solution, allowing for easy visualization of pH changes in bacteria cultures.
