The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
No,, the higher the phenol coefficient the stronger the disinfectant
A disinfectant with a phenol coefficient of 40 is highly effective compared to phenol as a standard. It means that it is 40 times more effective than phenol at killing microbes. This high coefficient indicates strong germicidal properties and efficiency in disinfecting surfaces.
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.
Because it's a test for phenols or enols
The phenol coefficient is a measure used to evaluate the disinfecting power of a chemical compared to phenol. It is determined by comparing the dilution of a test compound that achieves a specific level of microbial kill to that of phenol under identical conditions. A phenol coefficient greater than 1 indicates that the test compound is more effective than phenol, while a coefficient less than 1 indicates lower effectiveness. This metric helps in assessing the potency of disinfectants in microbiological applications.
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
No,, the higher the phenol coefficient the stronger the disinfectant
A disinfectant with a phenol coefficient of 40 is highly effective compared to phenol as a standard. It means that it is 40 times more effective than phenol at killing microbes. This high coefficient indicates strong germicidal properties and efficiency in disinfecting surfaces.
Molar extinction coefficient of phenol ret at 610nM is 22 mM-1 cm-1
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Phenol solution, or phenol directly from the manufacturer?If the phenol is in the form it comes in from the factory, no chemical test is necessary. Phenol is a white crystalline solid.If it's dissolved in water, the easiest test is pH. Phenol solution is more acidic than ethanol. So...whatever you use for a pH test will do ya.
No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.
Possible factors that can increase the absorbance of phenol in ethanol are: increasing the concentration of phenol in the solution, using a higher path length cuvette for measurement, and selecting a wavelength for measurement where phenol has a higher molar absorptivity coefficient.
because one bacterium takes 20 minuets to replicate.
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.