Because Potasium dichromate (Potassium Dichromate solvated in dilute (0.001N) perchlorate acid) gives absorption at two characteristics wavelength at 235nm to 350nm ( for 20mg/L to 100mg/L) and 430nm (for 600mg/L) that's why used in wide range for linerity test of spectrophotometer.
Because it shows characteristic known wavelengths at 235,257,313 and 430 at specified dilutions!
because KCl is transparent over the entire range of wavelengths (0.3 µm to 20 µm)
Potassium dichromate is very stable and can be obtained in a very pure form.
because its show characterestic absorbent
Cuz we dont know
how do you determine degree of deacetylation of chitosan using UV -vis spectrophotometer
about 7% of the UV rays hit the earth.
The continual interchange of oxygen between the atmosphere and the water, the plants and animals and mineral matter is called the oxygen cycle.
All parts of the atmosphere contain gases that absorb UV-B and more energetic radiation. All parts of our atmosphere pass UV-A.
Potassium dichromate is used as the primary standard for UV spectrophotometry because of its properties. It is pure, stable, has no waters of hydration, and has a high molar mass.
Cuz we dont know
The solution of potassium chloride is used to evaluate the stray light.
to check the sensitivity of the instrument
how do you determine degree of deacetylation of chitosan using UV -vis spectrophotometer
A 0.005M H2SO4 solution is used to prepare a K2Cr2O7 solution for UV calibration because it provides an acidic medium, which helps stabilize the dichromate Cr(VI) ions in the K2Cr2O7 solution. This stability ensures consistent and accurate UV calibration readings. Additionally, the specific concentration of H2SO4 is chosen to maintain a controlled environment for the reaction and analysis process.
Limit of stray light is important in UV calibration to ensure accurate measurements by reducing interference from unwanted light. This is typically done by using optical components that block or absorb stray light, such as filters or baffles. By minimizing stray light, the calibration process can be more precise and reliable.
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Holmium oxide is used as standard reference to check wavelengts.
Holmium oxide is used in calibration of UV spectrophotometers because it has well-defined absorption peaks at specific wavelengths in the UV range. These peaks can serve as reference points for calibrating the instrument and verifying its accuracy. Holmium oxide provides a reliable and stable reference for wavelength calibration in the UV spectrum.
Toluene has clear absorption peaks at 266 nm and 269 nm. Hexane is only the solvent.
If you think to potassium "permanganate" this compound can be decomposed under the influence of UV radiations.