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Q: Why use spectrophotometric methods for protein determination?
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What is protein sparer?

It means that the presence of the carbohydrates, spare the use of protein for energy use. Hence protein is use for body-building and not use for energy-giving.


How do you measurement calcium and phosphate using a spectrophotometer?

The appearance of kits for the determination of micro amounts of calcium stimulated our interest in the use of Eriochrome Blue SE for this analysis. A study of spectrophotometric curves indicates that, at pH values above 13.7, calcium will complex and cause a change of dye absorbance while magnesium does not complex with the dye. A differential spectrophotometric technic is described in this paper in which the spectrophotometer is set at zero absorbance with a dye-calcium standard solution or a dye-calcium sample solution as reference and the absorbance of the dye solution then measured. For a set of standards the absorbance-calcium relationship is linear. With the Beckman spectrophotometer, Model Dli, it has been found that 1 part of serum to 100 parts of alkaline dye solution (100 A of serum to 10 ml. alkaline dye solution) can be used. The technic shows greater sensitivity and accuracy than do previous methods using Eriochrome Blue SE.


The PI of protein is 9.24 At what PH MY protein binds to the column and which type of column have to use for purifification?

The PI of protein is 9.24 At what PH MY protein binds to the column and which type of column have to use for purification?


What methods besides weighing and measuring are use for charging systems?

subcooling methods


Blood cells that use protein to capture and carry oxygen is?

Blood cells use a protein called hemoglobin to capture oxygen.

Related questions

What can a determination do?

A determination can provide motivation, drive, and focus towards achieving a goal. It can help someone overcome obstacles, persevere through challenges, and stay committed to their objectives. Ultimately, determination can lead to success and personal growth.


How would you use determination in a sentence?

"His determination on the matter was second to none" etc.


Use the word determination in a sentence?

After much determination, she finally solved the problem.


Why is X-ray radiation required in protein structure determination?

Protein structure determination is basically crystallography i.e. finding out the arrangements of atoms. This technique uses Diffraction maxima and minima to determine 3D structures. For diffraction to occur, the size of the obstacle must be close to the wavelength of light used. Since the obstacles in this case are molecules, we use X rays, whose wavelength is comparable to radius of molecules.


How do you use the protein that you ingest?

you use the protein you ingest by using it for energy.


How would you use determination in a sentence with setting?

etc. It was shown through determination that I wanted to paint a good setting


What is protein sparer?

It means that the presence of the carbohydrates, spare the use of protein for energy use. Hence protein is use for body-building and not use for energy-giving.


How do you measurement calcium and phosphate using a spectrophotometer?

The appearance of kits for the determination of micro amounts of calcium stimulated our interest in the use of Eriochrome Blue SE for this analysis. A study of spectrophotometric curves indicates that, at pH values above 13.7, calcium will complex and cause a change of dye absorbance while magnesium does not complex with the dye. A differential spectrophotometric technic is described in this paper in which the spectrophotometer is set at zero absorbance with a dye-calcium standard solution or a dye-calcium sample solution as reference and the absorbance of the dye solution then measured. For a set of standards the absorbance-calcium relationship is linear. With the Beckman spectrophotometer, Model Dli, it has been found that 1 part of serum to 100 parts of alkaline dye solution (100 A of serum to 10 ml. alkaline dye solution) can be used. The technic shows greater sensitivity and accuracy than do previous methods using Eriochrome Blue SE.


What is percent recovery in protein purification?

When you use methods such as Solvent precipitation, you precipitate a protein molecule but this protein molecule normally does not have the same structure as that of protein in a solution (for example disulfide bonds do not connect the same amino acids) and it is not easy to change this to that of original structure even when the same solution is present. The percent of recovery means the percent that these denatured protein molecules can gain the same structure that they have in the solution before precipitation.


What methods did the mabo use to protest?

What methods did Eddie Mabo use to protest?


Does my kid really have ADD?

There is no single test to determine if your child has ADD. The first step in diagnosing ADD is to talk to your child's doctor or specialist. They will use various methods to make the determination as well as rule out any other problems.


How do they use redox titration in real-world application?

In the determination of iron in pharmaceutical preparations In the determination of iron in pharmaceutical preparations