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How is the redox agent DTT used in science?
DTT is a very strong redox agent. It can be used in enzyme stabilization. DTT has many uses in science including as an antioxidant. It is also used in the production of biofuel.
In protein separation why polyacrylamite is usedwhile in separation of DNA agarose is used?
Because proteins and protein fragements are often smaller than nucleic acid molecules. You can separate proteins on a modficated agarose gel (Metaphor by Lonza) made for the separation of smaller polynucleotids. http://www.lonzabioscience.com/Content/Documents/Bioscience/MetaPhor18108-0604-7.pdf Further to the answer above. Acrylamide is used because it can be used in conditions which contain SDS (sodium dodecyl sulphate) and DTT (a reducing agent). Both are necessary to resolve proteins by size. Proteins aren't uniformly charged, which will affect the way they migrate through a gel, but by coating them in SDS this gives them a uniform negative charge. DTT and SDS both help denature the protein and break up protein-protein and protein-DNA complexes thus allowing you to resolve the individual proteins.
What is SDS buffer?
SDS or sodiumdodecyl sulfate is a detergent used in protein separation. SDS buffer or SDS sample buffer consist of SDS, Tris, glycerol, bromo phenol blue, EDTA, and DTT or beta mercapto ethanol as a standard recipe. SDS is also added in stacking and separating gel preparation buffers that contain acrlamide.The main purpose is to keep the proteins denatured and provide the net negative charge to proteins as well as to run them according to it molecular weight
A reaction can be described using a word?
A reaction that can be described using a word
What is a sentence using the word monomer?
This is a sentence using the word monomer. :P
What is Laemmli gels?
It is the gel of choice for SDS PAGE
Who discovered SDS PAGE electrophoresis?
Laemmli U. K.
When was One Vision - DTT - created?
One Vision - DTT - was created in 2009.
How is the redox agent DTT used in science?
DTT is a very strong redox agent. It can be used in enzyme stabilization. DTT has many uses in science including as an antioxidant. It is also used in the production of biofuel.
What DTT is and describe how it affect environment?
stuff
A what concentration is DTT used in buffers?
1mM
Why is DTT hazardous?
because God made it that way
What makes DTT harmful over time?
it kills eagles
What is the function of dithiothreitol in DNA extraction?
DTT is the reducing agent for thiolated DNA
Can you get Air DT Max if you live in Lake Geneva?
No. But you can get Air DTT Max ITF11P
What is the function of DTT during DNA extraction?
Provides reducing conditions which prevent oxidation of the proteins cystidine residues
Who was the Bioligest who wrote aboutthe dangers of dtt?
In 1962, Rachel Carson wrote about the dangers of the pesticide DDT in her book The Silent Spring.
