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The test compound is not necessarily the ultimate carcinogen. In many cases the resulting products in metabolism rather than the compound itself causes cancer. Say, you feed a lab animal with a test compound and it gets cancer. You analyze the animal and you will find that the initial compound is metabolized to other chemicals that directly caused cancer. So this is why a test compound need to be metabolically activated, that is, it has to be metabolized by body enzymes (rat liver has a lot!) and then added to Salmonella to see the mutagenicity. Clear?
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Purpose of liver extract in the Ames test?
Ames test is used to test whether a certain substance is mutagenic. Liver extract is optionally added to simulate the effect of metabolism, as some compounds, like benzo[a]pyrene, are not mutagenic themselves but their metabolic products are.
What is the purpose of the ames test?
The Ames test identifies chemical mutagens.
What are the limitations of the Ames Test?
This test uses prokaryotes which means it isn't a perfect model for humans even after rat liver enzymes are added. Mutagens this test finds might also not be carcinogenic, meaning further tests are required. In addition not all carcinogens are mutagens (such as asbestos) meaning this test won't find them. Finally, some nitrate-containing substances can cause a false positive by production of nitric oxide. An example of this is nitroglycerin which is still widely used in treatment but gives a positive Ames result. Hope this is useful!
Can you reduce animal killing using AMES test?
not with ames but with animes
Are substances that test positive with the Ames test necessarily carciongenic in humans?
The substances that are test positive with Ames test may or may not be carcinogenic for humans. Similarly, some substances that cause cancer in laboratory animals do not give a positive result Ames test. It is not possible to decide the carcinogenicity of any substances only depending on the Ames test.
In what field of science would one carry out an Ames test?
Ames test would typically be carried out in the field of biology, more specifically toxicology. The Ames test involves testing genetics, which involves taking a sample and examining it closely in a laboratory.
What is the test used for detecting chemicals with carcinogenic potential?
The Ames Test
Describe the control component in an Ames Test?
The Ames test is a quick method that uses bacteria to assay for cancer causing chemicals. A false positive is possible when a nitrogen based material is used.
What is the purpose of an analanine aminotransferase test?
The alanine aminotransferase test (ALT) can reveal liver damage. It is probably the most specific test for liver damage. However, the severity of the liver damage is not necessarily shown by the ALT test.
When liver phosphates inc rice in lft test .?
infection of liver
Why are mutants used in ames test?
Mutants are not used, you have a culture of histidine dependent bacteria. This means that if you put them on a place with liver extract for food they should not make placks because there is no histidine pressent then you add a compound that you want to test if its a mutagen and if it is, you will start to see plaques form because the bacteria have mutated to becomes histadinde independent.
Your alt blood test is 70 is that really bad?
An alt blood test is a test of your liver enzymes. An alt of 70 shows mild to moderate fatty liver, and is not bad.
